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PMID: 6954522 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Stimulation of Xenopus oocyte protein synthesis by microinjected adenovirus RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 12 ·1982-06-00 ·Pages 3789-93

Richter JD, Jones NC, Smith LD

Abstract

The ability of injected heterologous mRNAs to compete with endogenous mRNAs in Xenopus oocytes was assayed. In confirmation of previous reports, globin mRNA translation in oocytes results in a concomitant decrease in endogenous protein synthesis. In contrast, injection of adenovirus 5 mRNA into the oocyte results in a stimulation of endogenous protein synthesis. The stimulation is dose dependent and does not require nuclear transcription in the oocyte. Preliminary mapping data suggest that the stimulatory RNA is a product of one of the viral immediate early genes.

MeSH Terms
Adenoviruses, Human/genetics Animals Egg Proteins/biosynthesis Female Gene Expression Regulation Genes, Viral Oocytes/metabolism Ovum/metabolism Poly A Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Time Factors Transcription, Genetic Xenopus laevis/genetics
Chemicals
Egg Proteins RNA, Messenger RNA, Viral Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Richter J D
Jones N C
Smith L D
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.12.3789
Published
1982-06-00
Pages
3789-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346513
Subset
IM
Grants
NICHD NIH HHS · HD-04229 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.12.3789 PMC Full Text View journal details
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