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PMID: 6952229 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Priming of DNA synthesis by diadenosine 5',5"'-P1,P4-tetraphosphate with a double-stranded octadecamer as a template and DNA polymerase alpha.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 6 ·1982-03-00 ·Pages 1791-4

Zamecnik PC, Rapaport E, Baril EF

Abstract

Diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) primes DNA synthesis in an in vitro system containing purified HeLa cell DNA polymerase alpha, deoxyadenosine triphosphate, and the double-stranded synthetic octadecamer template 5'-d-(G-G-A-G-G-C-T-T-T-T-T-T-G-G-A-G-G-C) (C-C-T-C-C-G-A-A-A-A-A-A-C-C-T-C-C-G)-d-5'; this octadecamer sequence is part of the origin region of DNA synthesis in simian virus 40. Ap4A is shown to be covalently linked to the first residue of the short deoxynucleotide chain synthesized under these experimental conditions. This template-primer system can initiate the new deoxynucleotide chain but cannot extend it beyond the A . T region.

MeSH Terms
Adenine Nucleotides/physiology Base Sequence DNA Replication DNA-Directed DNA Polymerase/metabolism Dinucleoside Phosphates HeLa Cells/enzymology Humans Structure-Activity Relationship Templates, Genetic
Chemicals
Adenine Nucleotides Dinucleoside Phosphates diadenosine tetraphosphate DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zamecnik P C
Rapaport E
Baril E F
References (21)
21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.6.1791
Published
1982-03-00
Pages
1791-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346066
Subset
IM
Grants
NCI NIH HHS · CA-15187 · United States
PHS HHS · P30-12708 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.6.1791 PMC Full Text View journal details
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