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PMID: 6947250 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Repair of O6-ethylguanine in DNA by a chromatin fraction from rat liver: transfer of the ethyl group to an acceptor protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 11 ·1981-11-00 ·Pages 6766-70

Mehta JR, Ludlum DB, Renard A, Verly WG

Abstract

Incubation of O6-[3H]ethylguanine-containing DNA with a rat liver chromatin fraction resulted in a decrease in the O6-ethylguanine content of the DNA. Analysis of the products of this reaction showed that the ethyl group had been transferred from the O6-ethylguanine to a protein acceptor. When the incubation mixture was separated on a cesium chloride gradient, the radioactivity removed from O6-ethylguanine appeared in a low-density band. This material has been isolated and subjected to trypsin digestion and high-pressure liquid chromatography analysis; it was sensitive to trypsin and the digest contained new high-pressure liquid chromatography peaks characteristic of oligopeptides. Radioactive peaks from the trypsin digestion have been digested further to the amino acid level and have been shown to contain S-[3H]ethylcysteine. Thus, we conclude that the repair activity in rat liver chromatin removes the ethyl group from O6-ethylguanine and transfers it to a cysteine moiety contained in an acceptor protein.

MeSH Terms

Animals Chromatin/metabolism Cysteine/analogs & derivatives,analysis DNA/biosynthesis DNA Repair Guanine/analogs & derivatives,metabolism Liver/metabolism Peptide Fragments/analysis Proteins/metabolism Rats

Chemicals

Chromatin Peptide Fragments Proteins 6-ethylguanine Guanine DNA S-ethylcysteine Cysteine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Mehta J R

Ludlum D B

Renard A

Verly W G

References (19)

19 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.11.6766

Published

1981-11-00

Pages

6766-70

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC349131

Subset

External Links

PubMed source DOI: 10.1073/pnas.78.11.6766 PMC Full Text View journal details

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Repair of O6-ethylguanine in DNA by a chromatin fraction from rat liver: transfer of the ethyl group to an acceptor protein.

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