Comparative studies of the hepatocarcinogen N,N-dimethylnitrosamine in vivo: reaction sites in rat liver DNA and the significance of their relative stabilities.

British journal of cancer ·Vol. 27 ·No. 2 ·1973-02-00 ·Pages 153-66

O'Connor PJ, Capps MJ, Craig AW

Abstract

The reaction of the hepatocarcinogen N,N-dimethylnitrosamine has been compared with that of methyl methanesulphonate, a methylating agent which is not a liver carcinogen. Consistent differences have been observed in the reaction of rat liver DNA in vivo with these agents; O(6)-alkylation and the production of unidentified acid-labile products were distinctive features of the reaction with the carcinogenic nitroso compound but were undetectable or in low yield, respectively, after reaction with the alkyl sulphonate. Evidence has been obtained for the excision of these reaction products in animals treated with the hepatocarcinogen and the significance of their relative stabilities is discussed.

MeSH Terms

Alkylation Animals Carbon Isotopes Chromatography Chromatography, Ion Exchange Chromatography, Paper DNA/isolation & purification Guanine/metabolism Hydrolysis Liver/drug effects,metabolism Male Methylation Nitrosamines/pharmacology Rats

Chemicals

Carbon Isotopes Nitrosamines Guanine DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

O'Connor P J

Capps M J

Craig A W

References (24)

24 references, click to expand

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Article Info

Journal

British journal of cancer

Abbr.

Br J Cancer

ISSN

0007-0920

DOI

10.1038/bjc.1973.19

Published

1973-02-00

Pages

153-66

Language

English

Region

England

NLM ID

0370635

PMCID

PMC2008841

Subset

External Links

PubMed source DOI: 10.1038/bjc.1973.19 PMC Full Text View journal details

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Comparative studies of the hepatocarcinogen N,N-dimethylnitrosamine in vivo: reaction sites in rat liver DNA and the significance of their relative stabilities.

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