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PMID: 6946501 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural and functional alterations in microtubule protein from Chinese hamster ovary cell mutants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 9 ·1981-09-00 ·Pages 5638-42

Keates RA, Sarangi F, Ling V

Abstract

We have examined mutant lines of Chinese hamster ovary cells that have increased resistance to the antimicrotubule drug Colcemid. Analysis of the functional properties of purified microtubule protein indicates that increased tolerance to the drug in vivo is reflected in altered properties of microtubules and tubulin in vitro. In this study, we have examined one series of related mutants and have found different microtubule alterations associated with each selection step. These changes include decreased Colcemid-binding affinity, an altered electrophoretic pattern of tubulin subcomponents, increased resistance to Colcemid inhibition of polymerization in vitro and, in one case, a decreased critical concentration for microtubule assembly. Characterized mutants of the class described here will be useful for probing the regulation of microtubule assembly in vivo.

MeSH Terms
Animals Cells, Cultured Cricetinae Cricetulus Demecolcine/pharmacology Drug Resistance Female Isoelectric Point Microtubules/physiology Mutation Ovary Protein Binding Tubulin/genetics
Chemicals
Tubulin Demecolcine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Keates R A
Sarangi F
Ling V
References (33)
33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.9.5638
Published
1981-09-00
Pages
5638-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348812
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.9.5638 PMC Full Text View journal details
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