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PMID: 269406 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Addition of colchicine--tubulin complex to microtubule ends: the mechanism of substoichiometric colchicine poisoning.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 8 ·1977-08-00 ·Pages 3466-70

Margolis RL, Wilson L

Abstract

Colchicine blocks microtubule polymerization by an unusual substoichiometric poisoning mechanism. We have investigated the mechanism by which this poisoning occurs with several experimental approaches, and have found that colchicine acts by addition to microtubule ends as a colchicine-tubulin complex.

MeSH Terms

Animals Brain/ultrastructure Cattle Chick Embryo Colchicine/pharmacology Glycoproteins/physiology Kinetics Microscopy, Electron Microtubules/drug effects,ultrastructure Tubulin/physiology Viscosity

Chemicals

Glycoproteins Tubulin Colchicine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Margolis R L

Wilson L

References (13)

13 references, click to expand

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Protein measurement with the Folin phenol reagent.
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Directionality of brain microtubule assembly in vitro.
Proc Natl Acad Sci U S A. 1974 May;71(5):1710-4 PMID: 4134974
The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein.
J Cell Biol. 1967 Aug;34(2):525-33 PMID: 6068183
Microtuble assembly: some possible regulatory mechanisms.
J Supramol Struct. 1974;2(2-4):429-50 PMID: 4612256
Biochemical studies on the in vitro assembly and disassembly of microtubules.
Ann N Y Acad Sci. 1975 Jun 30;253:133-46 PMID: 125062
The mechanism of action of colchicine. Colchicine binding properties of sea urchin sperm tail outer doublet tubulin.
J Cell Biol. 1973 Sep;58(3):709-19 PMID: 4747924
Characterization of microtubule assembly in porcine brain extracts by viscometry.
Biochemistry. 1973 Oct 9;12(21):4282-9 PMID: 4745672
Microtubule formation from two components separated by gel filtration of a tubulin preparation.
Biochim Biophys Acta. 1975 Jun 12;392(2):335-45 PMID: 805601
Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro.
Ann N Y Acad Sci. 1975 Jun 30;253:107-32 PMID: 1056738
Properties of tubulin in unfertilized sea urchin eggs. Quantitation and characterization by the colchicine-binding reaction.
J Cell Biol. 1976 Jun;69(3):599-607 PMID: 944700
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.8.3466

Published

1977-08-00

Pages

3466-70

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431611

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.8.3466 PMC Full Text View journal details

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Addition of colchicine--tubulin complex to microtubule ends: the mechanism of substoichiometric colchicine poisoning.

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