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PMID: 6941271 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The nucleotide sequence surrounding the replication terminus of R6K.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2095-9

Bastia D, Germino J, Crosa JH, Ram J

Abstract

The replication terminus of the plasmid R6K has been cloned into the single-stranded DNA phage vector M13mp5 and also into the plasmid vectors pBR313 and PBR322. By using single-stranded DNA templates prepared from the recombinant DNA clones, the sequence of 215 base pairs of DNA containing the replication terminus has been determined. The DNA sequence of the region of the terminus does not contain any 2-fold rotational symmetry. Therefore, folding of the DNA at the region of the terminus is unlikely to be a cause for replication termination. Interaction of a host-specified protein(s) with the sequence of the replication terminus is probably the basis of the mechanism of replicaion termination.

MeSH Terms
Bacterial Proteins/metabolism Bacteriophages/genetics Base Sequence Carrier Proteins/metabolism Cloning, Molecular DNA/metabolism DNA Replication DNA, Bacterial/genetics DNA-Binding Proteins Nucleic Acid Conformation Plasmids
Chemicals
Bacterial Proteins Carrier Proteins DNA, Bacterial DNA-Binding Proteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bastia D
Germino J
Crosa J H
Ram J
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2095
Published
1981-04-00
Pages
2095-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319290
Subset
IM
Grants
NIGMS NIH HHS · GM 24357 · United States
Databases
GENBANK
J01778
External Links
PubMed source DOI: 10.1073/pnas.78.4.2095 PMC Full Text View journal details
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