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PMID: 6938962 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Model for lactose repressor protein and its interaction with ligands.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 12 ·1980-12-00 ·Pages 7181-5

Dunaway M, Manly SP, Matthews KS

Abstract

A model is presented for the structure of the lactose repressor protein and for its interaction with inducer, operator DNA, and nonspecific DNA. The proposed structure is based on experimental evidence from this laboratory and from the literature and is offered as an integration of the available data on this system. Features unique to this model include: (i) interaction of the core region of the protein with the operator, (ii) primary effects of the conformational change in response to inducer on the core-operator interaction, (iii) contacts between all four subunits of the protein and the operator DNA, and (iv) qualitative differences in operator and nonspecific DNA binding.

MeSH Terms
Base Sequence Binding Sites DNA, Bacterial/metabolism Lac Operon Ligands Macromolecular Substances Models, Biological Protein Binding Repressor Proteins/metabolism Transcription Factors/metabolism
Chemicals
DNA, Bacterial Ligands Macromolecular Substances Repressor Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dunaway M
Manly S P
Matthews K S
References (45)
45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.12.7181
Published
1980-12-00
Pages
7181-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350465
Subset
IM
Grants
NIGMS NIH HHS · GM 22441-04 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.12.7181 PMC Full Text View journal details
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