Release of 1-O-alkylglyceryl 3-phosphorylcholine, O-deacetyl platelet-activating factor, from leukocytes: chemical ionization mass spectrometry of phospholipids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 12 ·1980-12-00 ·Pages 7019-23

Polonsky J, Tencé M, Varenne P, Das BC, Lunel J, Benveniste J

Abstract

Evidence is presented for the simultaneous release of platelet-activating factor (PAF-acether) and of its deacetylated derivative (lyso-PAF-acether) from hog leukocytes. On the basis of spectroscopy and chemical reactions, the structure of O-deacetyl-PAF is shown to be 1-O-alkylglyceryl 3-phosphorylcholine, an alkyl ether analog of lyso-phosphatidylcholine. Acetylation of lyso-PAF yields a compound with biological activity and chromatographical behavior indistinguishable from those of native PAF. Lyso-PAF may be considered to be either the precursor or the enzymatic degradation product of PAF. The usefulness of chemical ionization mass spectrometry for structural determination of phospholipids is also demonstrated.

MeSH Terms

Leukocytes/metabolism Lysophosphatidylcholines/metabolism Mass Spectrometry/methods Platelet Activating Factor/analogs & derivatives Platelet Aggregation/drug effects Structure-Activity Relationship

Chemicals

Lysophosphatidylcholines O-deacetyl platelet activating factor Platelet Activating Factor

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Polonsky J

Tencé M

Varenne P

Das B C

Lunel J

Benveniste J

References (20)

20 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.12.7019

Published

1980-12-00

Pages

7019-23

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC350432

Subset

External Links

PubMed source DOI: 10.1073/pnas.77.12.7019 PMC Full Text View journal details

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Release of 1-O-alkylglyceryl 3-phosphorylcholine, O-deacetyl platelet-activating factor, from leukocytes: chemical ionization mass spectrometry of phospholipids.

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