Genetic organization of the ribosomal transcription units of the yeast Saccharomyces carlsbergensis.

Nucleic acids research ·Vol. 5 ·No. 8 ·1978-08-00 ·Pages 2801-8

Meyerink JH, Retèl J, Raué HA, Planta RJ

Abstract

The genetic organization of the multiple ribosomal transcription units (RTUs) on the genome of the yeast Saccharomyces carlsbergensis was studied by electron microscopy of purified ribosomal DNA hybridized to 26S rRNA using the R-loop technique (Thomas, M., White, R.L. and Davis, R.W. (1973) Proc. Natl. Acad. Sci. U.S. 73, 2294-2298). Plasmid pBR 322, the molecular weight of which is known, was used as a standard for converting contour length of double-stranded DNA into molecular weight. The 140 yeast RTUs were found to be arrayed in tandem repeats, each repeat containing at most 0.4 X 10(6) D (about 6% of the length of the RTU) of non-transcribed spacer DNA. The repeats, in turn, are arranged in a number of clusters separated by much longer stretches of non-ribosomal DNA.

MeSH Terms

Genes Genetic Linkage Microscopy, Electron Molecular Weight Nucleic Acid Hybridization RNA, Ribosomal/genetics Saccharomyces/genetics Transcription, Genetic

Chemicals

RNA, Ribosomal

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Meyerink J H

Retèl J

Raué H A

Planta R J

References (16)

16 references, click to expand

The structural organization of ribosomal DNA in Drosophila melanogaster.
Cell. 1977 Feb;10(2):193-212 PMID: 402222
R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.
Cell. 1977 Feb;10(2):177-92 PMID: 402221
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Gene. 1977;2(2):95-113 PMID: 344137
Restriction endonuclease analysis of ribosomal DNA from Saccharomyces cerevisiae.
Mol Gen Genet. 1976 Nov 17;148(3):233-41 PMID: 796660
Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases. II. The physical map of EcoRI fragments.
Nucleic Acids Res. 1976 Oct;3(10):2697-707 PMID: 995649
Arrangement of length heterogeneity in repeating units of amplified and chromosomal ribosomal DNA from Xenopus laevis.
J Mol Biol. 1976 Aug 25;105(4):487-505 PMID: 972392
Hybridization of RNA to double-stranded DNA: formation of R-loops.
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2294-8 PMID: 781674
Properties of RNA in formamide.
Biochemistry. 1974 Dec 17;13(26):5367-73 PMID: 4611483
Nuclear satellite DNAs of yeast.
Biochim Biophys Acta. 1972 Oct 27;281(3):299-309 PMID: 4639276
Class of small multicopy plasmids originating from the mutant antibiotic resistance factor R1 drd-19B2.
J Bacteriol. 1975 Aug;123(2):658-65 PMID: 1097421
Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases.
Mol Biol Rep. 1976 Apr;2(5):393-400 PMID: 1083938
The heavy DNA satellite of yeast and its relationship to the ribosomal RNA genes.
Arch Biochem Biophys. 1976 May;174(1):187-91 PMID: 938051
The molecular weights of yeast ribosomal precursor RNAs.
Mol Biol Rep. 1975 Dec;2(4):321-5 PMID: 1214784
Characterization of yeast ribosomal DNA.
Eur J Biochem. 1975 Oct 1;58(1):51-7 PMID: 1183440
The size and the location of the ribosomal RNA segments in ribosomal precursor RNA of yeast.
Biochim Biophys Acta. 1971 Mar 25;232(3):494-508 PMID: 5572619
On the mechanism of the biosynthesis of ribosomal RNA in yeast.
Biochim Biophys Acta. 1970 Dec 14;224(2):458-69 PMID: 5498077

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.8.2801

Published

1978-08-00

Pages

2801-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342208

Subset

External Links

PubMed source DOI: 10.1093/nar/5.8.2801 PMC Full Text View journal details

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Genetic organization of the ribosomal transcription units of the yeast Saccharomyces carlsbergensis.

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