The molecular weights of yeast ribosomal precursor RNAs.

Molecular biology reports ·Vol. 2 ·No. 4 ·1975-12-00 ·Pages 321-5

Brand RC, Planta RJ

Abstract

The molecular weights of the predominant rRNA precursors as well as those of 26-S and 17-S mature rRNA from Saccharomyces carlsbergensis were determined by polyacrylamide gel electrophoresis in the presence of formamide. Mature 26-S + 5.8-S rRNA was found to have a molecular weight of 1.24 X 10(6) while their immediate precursor, 29-S RNA, had a molecular weight of 1.52 X 10(6). Values of 0.70 X 10(6) and 0.82 X 10(6) were obtained for the molecular weights of mature 17-S rRNA and its 18-S precursor. Finally the 37-S precursor, common to both 29-S and 18-S RNA, was found to have a molecular weight of 2.80 X 10(6). Each precursor rRNA, therefore, contains extra sequences not found at the next stage of maturation.

MeSH Terms

Electrophoresis, Polyacrylamide Gel Molecular Weight Nucleic Acid Denaturation RNA, Ribosomal/biosynthesis Saccharomyces/metabolism

Chemicals

RNA, Ribosomal

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Brand R C

Planta R J

References (18)

18 references, click to expand

Ribosomal precursor particles from yeast.
Exp Cell Res. 1975 Jan;90(1):95-104 PMID: 1122947
Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli.
Biochemistry. 1965 Jul;4(7):1302-11 PMID: 5323136
Molecular weight determination of nucleic acids by gel electrophoresis in non-aqueous solution.
Nat New Biol. 1972 Jan 26;235(56):108-10 PMID: 4551278
Structural comparison of 37-S and 32-S ribosomal precursor RNA in yeast with the mature ribosomal RNA components.
Biochim Biophys Acta. 1973 Feb 4;294(1):464-71 PMID: 4705995
Molecular weights of plant viral RNAs determined by gel electrophoresis under denaturing conditions.
Virology. 1974 Aug;60(2):515-21 PMID: 4367491
On the mechanism of the biosynthesis of ribosomal RNA in yeast.
Biochim Biophys Acta. 1970 Dec 14;224(2):458-69 PMID: 5498077
Gel electrophoresis of RNA under denaturing conditions.
Biochim Biophys Acta. 1973 Oct 26;324(3):320-33 PMID: 4202717
Electrophoresis of RNA in formamide.
Biochemistry. 1974 Dec 17;13(26):5373-8 PMID: 4611484
Properties of the ribosomal RNA precursor in Xenopus laevis; comparison to the precursor in mammals and in plants.
J Mol Biol. 1969 Oct 28;45(2):353-66 PMID: 5367033
Ribosomal precursor RNA in Saccharomyces carlsbergensis.
Eur J Biochem. 1967 Dec;3(2):248-58 PMID: 6082612
The investigation of the ribosomal RNA sites in yeast DNA by the hybridization technique.
Biochim Biophys Acta. 1968 Dec 17;169(2):416-29 PMID: 5750457
Effect of infection with T-even phage on the inducible synthesis of beta-glactosidase in Escherichia coli.
J Mol Biol. 1967 Aug 14;27(3):453-68 PMID: 4860579
Lysis of Escherichia coli with a neutral detergent.
Biochim Biophys Acta. 1967 Dec 19;149(2):476-88 PMID: 4966087
The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.
J Biol Chem. 1973 Jun 10;248(11):3860-75 PMID: 4575197
The effect of temperature and ionic strength of the electrophoretic mobility of yeast mitochondrial RNA.
Eur J Biochem. 1971 Mar 1;19(1):64-72 PMID: 4927999
Properties of RNA in formamide.
Biochemistry. 1974 Dec 17;13(26):5367-73 PMID: 4611483
Primary sequence of the 16S ribosomal RNA of Escherichia coli.
Nucleic Acids Res. 1975 Feb;2(2):265-78 PMID: 1091918
Structural comparison of 17 S ribosomal RNA of yeast and its immediate precursor, 18 S RNA.
FEBS Lett. 1972 Jul 15;24(1):93-7 PMID: 5086624

Article Info

Journal

Molecular biology reports

Abbr.

Mol Biol Rep

ISSN

0301-4851

DOI

10.1007/BF00357019

Published

1975-12-00

Pages

321-5

Language

English

Region

Netherlands

NLM ID

0403234

Subset

External Links

PubMed source DOI: 10.1007/BF00357019 View journal details

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The molecular weights of yeast ribosomal precursor RNAs.

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