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PMID: 6893201 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Contractile basis of ameboid movement. VII. Aequorin luminescence during ameboid movement, endocytosis, and capping.

The Journal of cell biology ·Vol. 86 ·No. 2 ·1980-08-00 ·Pages 599-607

Taylor DL, Blinks JR, Reynolds G

Abstract

Aequorin luminescence has been utilized to determine the spatial and temporal fluctuations of the free calcium ion concentration [Ca++] in Chaos carolinensis during ameboid movement, pinocytosis, and capping. The [Ca++] increases above approximately 10(-7) M during normal ameboid movement. Three types of luminescent signals are detected in cells: continuous luminescence, spontaneous pulses, and stimulated pulses. Continuous luminescence is localized in the tails of actively motile cells, and spontaneous pulses occur primarily over the anterior regions of cells. We are sometimes able to correlate the spontaneous pulses with extending pseudopods, whereas stimulated pulses are induced by mechanical damage, electrical stimulation, concanavalin A-induced capping, and pinocytosis. The localization of both distinct actin structures and sites where [Ca++] increases suggests cellular sites of contractile activity. The independent evidence from localizing actin structures and the distribution of [Ca++] can also be viewed in relation to the solation-contraction coupling hypothesis defined in vitro.

MeSH Terms
Actins/metabolism Aequorin Amoeba/physiology Animals Calcium/metabolism Cell Membrane/physiology Cytoskeleton/physiology,ultrastructure Endocytosis Movement Receptors, Concanavalin A/physiology
Chemicals
Actins Receptors, Concanavalin A Aequorin Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Taylor D L
Blinks J R
Reynolds G
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52 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.86.2.599
Published
1980-08-00
Pages
599-607
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111474
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.86.2.599 PMC Full Text View journal details
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