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PMID: 68472 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Terminal redundancy and the origin of replication of Rous sarcoma virus RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 5 ·1977-05-00 ·Pages 1908-12

Coffin JM, Haseltine WA

Abstract

In vitro synthesis of Rous sarcoma virus DNA by the virion endogenous DNA polymerase activity is initiated on a tRNAtrp primer located near the 5' end of the genome. A major product of such synthesis is a piece of DNA 101 nucleotides long (strong stop DNA) which can be isolated covalently bound to the tRNA primer. Here we show that the strong stop DNA is complementary to the extreme 5' end of the genome. We also show that the 5' and 3' termini of the Rous sarcoma virus genome, excluding the cap and the poly(A), have the identical sequence. We propose that the function of this sequence is to facilitate elongation from the 3' end of DNA chains initiated elsewhere on the virus genome.

MeSH Terms
Avian Sarcoma Viruses/metabolism Base Sequence Chromosome Mapping DNA, Viral/biosynthesis Nucleic Acid Hybridization Poly A/metabolism RNA, Transfer/metabolism RNA, Viral/metabolism RNA-Directed DNA Polymerase/metabolism Templates, Genetic Virus Replication
Chemicals
DNA, Viral RNA, Viral Poly A RNA, Transfer RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coffin J M
Haseltine W A
References (22)
22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.5.1908
Published
1977-05-00
Pages
1908-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431041
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.5.1908 PMC Full Text View journal details
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