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PMID: 6824758 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Theoretical analysis of fluorescence photobleaching recovery experiments.

Biophysical journal ·Vol. 41 ·No. 1 ·1983-01-00 ·Pages 95-7

Soumpasis DM

Abstract

We derive an exact closed formula for the fluorescence recovery curve measured in fluorescence photobleaching recovery experiments employing uniform circular laser beams. In contrast to the expression used currently, this result is very simple and free of mathematical drawbacks, thus facilitating the quantitative analysis of experimental data.

MeSH Terms
Mathematics Membranes/analysis Models, Biological Photochemistry Spectrometry, Fluorescence/methods
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Soumpasis D M
References (6)
6 references, click to expand
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    Biophys J. 1976 Sep;16(9):1055-69 PMID: 786399
  2. Dynamics of fluorescence marker concentration as a probe of mobility.
    Biophys J. 1976 Nov;16(11):1315-29 PMID: 974223
  3. Determination of molecular motion in membranes using periodic pattern photobleaching.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2759-63 PMID: 275845
  4. Comparative lateral diffusion of fluorescent lipid analogues in phospholipid multibilayers.
    Biochemistry. 1980 Dec 23;19(26):6050-7 PMID: 7470449
  5. Translational mobility of glycophorin in bilayer membranes of dimyristoylphosphatidylcholine.
    Biochemistry. 1981 Mar 3;20(5):1392-6 PMID: 7225336
  6. Fluorescence redistribution after photobleaching. A new multipoint analysis of membrane translational dynamics.
    Biophys J. 1979 Nov;28(2):281-91 PMID: 262551
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(83)84410-5
Published
1983-01-00
Pages
95-7
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1329018
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(83)84410-5 PMC Full Text View journal details
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