Home LiteratureArticle Details
PMID: 262551 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Fluorescence redistribution after photobleaching. A new multipoint analysis of membrane translational dynamics.

Biophysical journal ·Vol. 28 ·No. 2 ·1979-11-00 ·Pages 281-91

Koppel DE

Abstract

A theoretical formulation and experimental methodology are presented for a new multipoint analysis of membrane translational dynamics. The redistribution of fluorescent probe after a localized photobleaching pulse is monitored at several locations by a focused laser beam sequentially scanned through the bleached area. The spatial information so obtained provides a unique sensitivity to possible systematic flow and a direct internal calibration of the characteristic transport distance. These capabilities are demonstrated with experimental data on a reconstituted multibilayer system.

MeSH Terms
Cell Membrane/physiology Kinetics Mathematics Models, Biological Molecular Conformation Photolysis Spectrometry, Fluorescence/instrumentation
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Koppel D E
References (21)
21 references, click to expand
  1. Lateral diffusion of a hydrophobic peptide, N-4-nitrobenz-2-oxa-1,3-diazole gramicidin S, in phospholipid multibilayers.
    Biochemistry. 1978 Dec 12;17(25):5543-50 PMID: 83159
  2. Determination of molecular motion in membranes using periodic pattern photobleaching.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2759-63 PMID: 275845
  3. Lateral diffusion in plasma membrane of mouse egg is restricted after fertilisation.
    Nature. 1978 Mar 30;272(5652):448-50 PMID: 345127
  4. Receptor diffusion on cell surfaces modulated by locally bound concanavalin A.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1110-4 PMID: 265557
  5. Lateral transport of a lipid probe and labeled proteins on a cell membrane.
    Science. 1977 Jan 21;195(4275):307-9 PMID: 556653
  6. Diffusion and patching of macromolecules on planar lipid bilayer membranes.
    Biochemistry. 1977 Jul 26;16(15):3476-83 PMID: 560860
  7. Lateral diffusion in phospholipid bilayer membranes and multilamellar liquid crystals.
    Biochemistry. 1978 Jul 25;17(15):3046-53 PMID: 698183
  8. Control of acetylcholine receptor mobility and distribution in cultured muscle membranes. A fluorescence study.
    Biochim Biophys Acta. 1978 Jul 20;511(1):23-38 PMID: 667056
  9. Measurement of the lateral mobility of cell surface components in single, living cells by fluorescence recovery after photobleaching.
    J Supramol Struct. 1976;5(4):565(417)-576(428) PMID: 800621
  10. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
    Biophys J. 1976 Sep;16(9):1055-69 PMID: 786399
  11. Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4594-8 PMID: 1070010
  12. Dynamics of fluorescence marker concentration as a probe of mobility.
    Biophys J. 1976 Nov;16(11):1315-29 PMID: 974223
  13. Rotational and translational diffusion in membranes.
    Annu Rev Biophys Bioeng. 1974;3(0):179-201 PMID: 4371655
  14. Microviscosity parameters and protein mobility in biological membranes.
    Biochim Biophys Acta. 1976 Apr 16;433(1):133-49 PMID: 1260056
  15. Measurement of the translational mobility of concanavalin A in glycerol-saline solutions and on the cell surface by fluorescence recovery after photobleaching.
    Biochim Biophys Acta. 1976 Apr 16;433(1):215-22 PMID: 177080
  16. A microfluorimetric study of translational diffusion in erythrocyte membranes.
    Biochim Biophys Acta. 1974 Nov 15;367(3):282-94 PMID: 4429678
  17. Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2409-13 PMID: 1065895
  18. Surface modulation in cell recognition and cell growth.
    Science. 1976 Apr 16;192(4236):218-26 PMID: 769162
  19. Directed lipid flow in cell membranes.
    Nature. 1976 Mar 4;260(5546):21-3 PMID: 1264188
  20. Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over surface components.
    Biochim Biophys Acta. 1976 Apr 13;457(1):57-108 PMID: 1260065
  21. Measurement of membrane protein lateral diffusion in single cells.
    Science. 1976 Feb 6;191(4226):466-8 PMID: 1246629
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(79)85176-0
Published
1979-11-00
Pages
281-91
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1328630
Subset
IM
Grants
NIGMS NIH HHS · GM23585 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(79)85176-0 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2