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PMID: 6823011 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence arrangement and protein coding capacity of the adenovirus type 2 "i" leader.

Journal of virology ·Vol. 45 ·No. 1 ·1983-01-00 ·Pages 185-91

Falvey E, Ziff E

Abstract

The adenovirus type 2 (Ad-2) "i" leader is an RNA segment which is preserved in some mRNA species from the Ad-2 late transcription unit. It maps between the second and third segments of the standard tripartite leader. We located the boundaries of the i leader in genomic Ad-2 DNA and determined its nucleotide sequence. The leader contains an ATG initiator near its 5' boundary, followed by a reading frame which is open for translation. We suggest that the i leader constitutes an Ad-2 coding sequence whose novel position within the leader of major late transcription unit messengers allows it to be translated in preference to coding sequences in mRNA main bodies. The i leader potentially contributes to the coding sequences of a family of proteins. Also, a Northern blot analysis of late mRNAs containing the i leader suggests that it may be retained in the leaders of many different late transcription unit mRNAs. We compare the i leader to the simian virus 40 agnogene.

MeSH Terms
Adenoviruses, Human/genetics Amino Acid Sequence Base Sequence Codon DNA, Viral/genetics Genes, Viral Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Transcription, Genetic Viral Proteins/genetics
Chemicals
Codon DNA, Viral RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Falvey E
Ziff E
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23 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.45.1.185-191.1983
Published
1983-01-00
Pages
185-91
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256400
Subset
IM
Databases
GENBANK
J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946
External Links
PubMed source DOI: 10.1128/JVI.45.1.185-191.1983 PMC Full Text View journal details
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