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PMID: 6281734 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An adenovirus agnogene.

Nucleic acids research ·Vol. 10 ·No. 8 ·1982-04-24 ·Pages 2539-48

Virtanen A, Aleström P, Persson H, Katze MG, Pettersson U

Abstract

The nucleotide sequence of a 550 base pairs long segment, located between map positions 21 and 22.5 in the adenovirus type 2 genome has been determined. S1 nuclease mapping and sequence analysis of cDNA copies of adenovirus mRNA demonstrated that the established sequence includes the i-leader which is spliced to the 5'-end of certain adenovirus mRNAs (1). The i-leader which is 440 nucleotides long contains an open translational reading frame which is preceded by an AUG triplet and which terminates in the third segment of the tripartite leader. A polypeptide with the same molecular weight as predicted from the DNA sequence was identified by in vitro translation of mRNA which had been selected by hybridization to DNA fragments, containing sequences from the i-leader. The results thus suggest that the i-leader, unlike other adenovirus leader segments, is used for translation.

MeSH Terms

Adenoviruses, Human/genetics Amino Acid Sequence Base Composition Base Sequence DNA/metabolism DNA Restriction Enzymes DNA, Viral/genetics Genes, Viral Molecular Weight Protein Biosynthesis RNA, Messenger/genetics Transcription, Genetic

Chemicals

DNA, Viral RNA, Messenger DNA DNA Restriction Enzymes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Virtanen A

Aleström P

Persson H

Katze M G

Pettersson U

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24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.8.2539

Published

1982-04-24

Pages

2539-48

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320632

Subset

Databases

GENBANK

J01917, J01918, J01919, J01920, J01921, J01922, J01923, J01924, J01925, J01926, J01927, J01928, J01929, J01930, J01931, J01932, J01933, J01934, J01935, J01936, J01937, J01938, J01939, J01940, J01941, J01942, J01943, J01944, J01945, J01946

External Links

PubMed source DOI: 10.1093/nar/10.8.2539 PMC Full Text View journal details

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