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PMID: 6822666 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Z-DNA-specific antibodies in human systemic lupus erythematosus.

The Journal of clinical investigation ·Vol. 71 ·No. 2 ·1983-02-00 ·Pages 314-21

Lafer EM, Valle RP, Möller A, Nordheim A, Schur PH, Rich A, Stollar BD

Abstract

Naturally occurring antibodies to left-handed Z-DNA have been shown to be present in the sera of human patients with systemic lupus erythematosus (SLE). These antibodies are of two types. One type reacts with both denatured DNA and Z-DNA. The other type is specific for Z-DNA and remained in the serum after removal of the cross-reactive antibody by extensive absorption on a denatured DNA affinity column. The antibodies appear to be specific for SLE and do not appear frequently in other rheumatic diseases. The presence of the antibody in SLE is correlated with the clinical manifestations of the disease, in parallel with antibodies to native and denatured DNA.

MeSH Terms
Antibody Specificity Autoantibodies/classification,isolation & purification Chromatography, Agarose DNA/immunology Humans Lupus Erythematosus, Systemic/immunology Nucleic Acid Conformation Osmolar Concentration Radioimmunoassay
Chemicals
Autoantibodies DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lafer E M
Valle R P
Möller A
Nordheim A
Schur P H
Rich A
Stollar B D
References (22)
22 references, click to expand
  1. Antibodies to ribosomal ribonucleic acid (rRNA) in patients with systemic lupus erythematosus (SLE).
    Immunology. 1970 Sep;19(3):439-42 PMID: 4097261
  2. Immune complex systems in the nephritis of systemic lupus erythematosus.
    Kidney Int. 1973 Feb;3(2):90-9 PMID: 4121101
  3. Antinucleolar antibodies in human sera.
    J Immunol. 1973 Oct;111(4):996-1004 PMID: 4199560
  4. Antinuclear antibodies (ANA): immunologic and clinical significance.
    Semin Arthritis Rheum. 1976 Nov;6(2):83-124 PMID: 62398
  5. Naturally-occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus.
    Nature. 1977 Jan 13;265(5590):175-7 PMID: 834260
  6. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  7. The reaction of SLE antibodies with native, single stranded RNA: radioassay and binding specificities.
    J Immunol. 1978 Feb;120(2):550-7 PMID: 340588
  8. Recent progress in the study of autoantibodies to nuclear antigens.
    Hum Pathol. 1978 Jan;9(1):85-91 PMID: 344192
  9. Loss of suppressor T-lymphocyte function in patients with systemic lupus erythematosus (SLE).
    Clin Exp Immunol. 1978 Apr;32(1):125-33 PMID: 352583
  10. Autoimmunity and the immunologic network.
    Arthritis Rheum. 1978 Sep-Oct;21(7):853-61 PMID: 212088
  11. Immunoregulatory aberrations in systemic lupus erythematosus.
    J Immunol. 1978 Oct;121(4):1473-9 PMID: 359708
  12. Molecular structure of a left-handed double helical DNA fragment at atomic resolution.
    Nature. 1979 Dec 13;282(5740):680-6 PMID: 514347
  13. Reaction of systemic lupus erythematosus antinative DNA antibodies with native DNA fragments from 20 to 1,200 base pairs.
    J Clin Invest. 1980 Feb;65(2):469-77 PMID: 6153184
  14. Secondary structure in denatured DNA is responsible for its reaction with antinative DNA antibodies of systemic lupus erythematosus sera.
    J Clin Invest. 1980 Aug;66(2):210-9 PMID: 6156950
  15. Left-handed double helical DNA: variations in the backbone conformation.
    Science. 1981 Jan 9;211(4478):171-6 PMID: 7444458
  16. Left-handed DNA in restriction fragments and a recombinant plasmid.
    Nature. 1981 Apr 23;290(5808):672-7 PMID: 6261149
  17. Immune complexes, complement, and anti-DNA in exacerbations of systemic lupus erythematosus (SLE).
    Medicine (Baltimore). 1981 May;60(3):208-17 PMID: 7231154
  18. Polyspecific monoclonal lupus autoantibodies reactive with both polynucleotides and phospholipids.
    J Exp Med. 1981 Apr 1;153(4):897-909 PMID: 6972993
  19. Antibodies specific for left-handed Z-DNA.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3546-50 PMID: 6943554
  20. Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes.
    Nature. 1981 Dec 3;294(5840):417-22 PMID: 6796893
  21. Flipping of cloned d(pCpG)n.d(pCpG)n DNA sequences from right- to left-handed helical structure by salt, Co(III), or negative supercoiling.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4560-4 PMID: 6956879
  22. Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding.
    Cell. 1982 Dec;31(2 Pt 1):309-18 PMID: 7159926
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/jci110771
Published
1983-02-00
Pages
314-21
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC436869
Subset
IM
Grants
NIADDK NIH HHS · AM05577 · United States
NIADDK NIH HHS · AM11414 · United States
NIADDK NIH HHS · AM27232 · United States
External Links
PubMed source DOI: 10.1172/jci110771 PMC Full Text View journal details
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