Home LiteratureArticle Details
PMID: 6813825 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Replication of the broad host range plasmid RSF1010 in cell-free extracts of Escherichia coli and Pseudomonas aeruginosa.

Nucleic acids research ·Vol. 10 ·No. 15 ·1982-08-11 ·Pages 4687-702

Diaz R, Staudenbauer WL

Abstract

Replication of exogenous RSF1010 DNA can be carried out by soluble enzyme systems from Escherichia coli and Pseudomonas aeruginosa. It requires the function of RSF1010-encoded replication protein(s), which is not expressed in extracts from plasmid-free bacteria. In contrast to previously described in vitro systems for plasmid replication, initiation of RSF1010 DNA synthesis is independent of transcription catalyzed by host RNA polymerase. This is indicated by the insensitivity of RSF1010 replication to rifampicin as well as to RNA polymerase antibodies. It is proposed that a host RNA polymerase transcription-independent initiation mechanism might be a general property of broad host range plasmids.

MeSH Terms
Anti-Bacterial Agents/pharmacology DNA Replication/drug effects DNA-Directed RNA Polymerases/metabolism Escherichia coli/drug effects,genetics Kinetics Mutation Plasmids Pseudomonas aeruginosa/drug effects,genetics Species Specificity Transcription, Genetic
Chemicals
Anti-Bacterial Agents DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Diaz R
Staudenbauer W L
References (22)
22 references, click to expand
  1. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.
    Gene. 1981 Dec;16(1-3):237-47 PMID: 6282695
  2. Inhibition of RNA polymerase by streptolydigin.
    Nat New Biol. 1971 Apr 14;230(15):197-200 PMID: 5280166
  3. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  4. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  5. Molecular nature of two nonconjugative plasmids carrying drug resistance genes.
    J Bacteriol. 1974 Feb;117(2):619-30 PMID: 4590480
  6. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  7. phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3907-11 PMID: 670
  8. Effects of different alleles of the E. coli K12 pol A gene on the replication of non-transferring plasmids.
    Mol Gen Genet. 1976 Feb 2;143(3):311-8 PMID: 765763
  9. Replication of small plasmids in extracts of Escherichia coli.
    Mol Gen Genet. 1976 Jun 15;145(3):273-80 PMID: 781516
  10. Characterization of SmSu plasmids by restriction endonuclease cleavage and compatibility testing.
    J Bacteriol. 1976 Oct;128(1):394-400 PMID: 789344
  11. REPLICAtion of small plasmids in extracts of Escherichia coli: requirement for both DNA polymerases I and II.
    Mol Gen Genet. 1976 Dec 8;149(2):151-8 PMID: 796676
  12. Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.
    J Bacteriol. 1978 Jun;134(3):1117-22 PMID: 350840
  13. Replication of antibiotic resistance plasmid R6K DNA in vitro.
    Biochemistry. 1978 Jun 27;17(13):2567-73 PMID: 354690
  14. Requirement of a plasmid-encoded protein for replication in vitro of plasmid R6K.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5381-5 PMID: 364478
  15. Characterization of an improved in vitro DNA replication system for Escherichia coli plasmids.
    Nucleic Acids Res. 1979 Jul 25;6(10):3289-304 PMID: 384367
  16. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  17. Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2450-4 PMID: 6156450
  18. Plasmid R1 DNA replication dependent on protein synthesis in cell-free extracts of E. coli.
    Nature. 1981 Jan 22;289(5795):326-8 PMID: 7005697
  19. Plasmid RP4 specifies a deoxyribonucleic acid primase involved in its conjugal transfer and maintenance.
    J Bacteriol. 1981 Dec;148(3):769-81 PMID: 6273381
  20. Discriminatory function of ribonuclease H in the selective initiation of plasmid DNA replication.
    Nucleic Acids Res. 1982 Feb 11;10(3):833-53 PMID: 6278438
  21. Enzymatic replication of the origin of the Escherichia coli chromosome.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7370-4 PMID: 6278471
  22. Replication of lambda dv DNA in vitro.
    Nucleic Acids Res. 1982 Mar 11;10(5):1733-40 PMID: 6461846
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.15.4687
Published
1982-08-11
Pages
4687-702
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321122
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.15.4687 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2