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PMID: 350840 Published · ppublish English Journal Article

Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12.

Journal of bacteriology ·Vol. 134 ·No. 3 ·1978-06-00 ·Pages 1117-22

de Graaff J, Crosa JH, Heffron F, Falkow S

Abstract

Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI ends. Replication proceeded either unidirectionally or bidirectionally with equal frequency. Results of the analysis of replicative intermediates of RSF1010 containing the Apr-transposable sequence (Tn) are also presented.

MeSH Terms
DNA Replication DNA Restriction Enzymes/metabolism DNA, Bacterial/analysis,biosynthesis DNA, Circular/analysis,biosynthesis Escherichia coli/analysis,genetics,metabolism R Factors
Chemicals
DNA, Bacterial DNA, Circular DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
de Graaff J
Crosa J H
Heffron F
Falkow S
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26 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.134.3.1117-1122.1978
Published
1978-06-00
Pages
1117-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222362
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.134.3.1117-1122.1978 PMC Full Text View journal details
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