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PMID: 6811749 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Review

Amino acid transport in isolated rat hepatocytes.

The Journal of membrane biology ·Vol. 69 ·No. 1 ·1982-00-00 ·Pages 1-12

Kilberg MS

Abstract

Improvements in the collagenase perfusion techniques have made isolated rat hepatocytes a popular model in which to study hepatic function. Our knowledge of hepatic amino acid transport has been advanced as a result of this methodology. Translocation across the hepatocyte plasma membrane can, in some instances, represent the rate-limiting step in the overall metabolism of certain amino acids. Furthermore, regulation of amino acid uptake by hepatocytes appears to play a role in diabetes, and perhaps in malignant transformation. Comparisons between normal adult hepatocytes and several hepatoma cell lines show basic differences in amino acid transport. There are at least eight distinct systems in normal hepatocytes for transport of the hormones. Systems A and N exhibit enhanced uptake rates after the cells have been maintained in the absence of extracellular amino acids, a phenomenon termed adaptive control. Further studies using isolated hepatocytes will increase our basic understanding of membrane transport processes and their regulation.

MeSH Terms
Amino Acids/metabolism Aminoisobutyric Acids/metabolism Animals Biological Transport/drug effects Catecholamines/pharmacology Cell Membrane/metabolism Cells, Cultured Cysteine/metabolism Glucagon/pharmacology Glucocorticoids/pharmacology Glutamine/metabolism Glycine/metabolism Histidine/metabolism In Vitro Techniques Insulin/pharmacology Kinetics Liver/drug effects,metabolism Lysine/metabolism Rats
Chemicals
Amino Acids Aminoisobutyric Acids Catecholamines Glucocorticoids Insulin Glutamine 2-(methylamino)isobutyric acid Histidine Glucagon Lysine Cysteine Glycine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kilberg M S
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01871236
Published
1982-00-00
Pages
1-12
Language
English
Region
United States
NLM ID
0211301
Subset
IM
Grants
NIADDK NIH HHS · AM-28374 · United States
External Links
PubMed source DOI: 10.1007/BF01871236 View journal details
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