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PMID: 13365 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

3':5'-cyclic AMP: independent induction of amino acid transport by epinephrine in primary cultures of adult rat liver cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 1 ·1977-01-00 ·Pages 234-7

Pariza MW, Butcher FR, Becker JE, Potter VR

Abstract

Liver cells were obtained from adult rats by a collagenase perfusion technique and cultured as monolayers in serum-free media. Epinephrine and isoproterenol both induced large increases in intracellular adenosine 3':5'-monophosphate (cAMP) within 1-2 min whereas epinephrine (but not isoproterenol) induced 2- to 3-fold increases in the rate of alpha-aminoisobutyric acid transport within 2-4 hr after a 1 hr lag. Propranolol abolished the increase in cAMP elicited by epinephrine and isoproterenol, but did not block the induction of alpha-aminoisobutyric acid transport by epinephrine. In contrast, dihydroergotamine abolished and phentolamine diminished the induction of alpha-aminoisobutyric acid transport by epinephrine but did not decrease the stimulation of cAMP levels by epinephrine. Epinephrine dose response curves for cAMP and alpha-aminoisobutyric acid transport were similar. Once exposed to epinephrine, cells became refractory to further stimulation of cAMP levels by epinephrine.

MeSH Terms

Adrenergic alpha-Antagonists/pharmacology Aminoisobutyric Acids/metabolism Animals Biological Transport/drug effects Cells, Cultured Cyclic AMP/metabolism Dose-Response Relationship, Drug Epinephrine/pharmacology Isoproterenol/pharmacology Kinetics Liver/metabolism Rats

Chemicals

Adrenergic alpha-Antagonists Aminoisobutyric Acids Cyclic AMP Isoproterenol Epinephrine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Pariza M W

Butcher F R

Becker J E

Potter V R

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21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.74.1.234

Published

1977-01-00

Pages

234-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC393233

Subset

External Links

PubMed source DOI: 10.1073/pnas.74.1.234 PMC Full Text View journal details

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3':5'-cyclic AMP: independent induction of amino acid transport by epinephrine in primary cultures of adult rat liver cells.

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