The importance of the production of phosphatidic acid for the release of arachidonic acid in stimulated platelets.

Agents and actions ·Vol. 11 ·No. 6-7 ·1981-12-00 ·Pages 536-7

Lapetina EG, Billah MM

Abstract

Phosphatidic acid is formed by platelets as a result of the combined activities of phospholipase C and diacylglycerol kinase. The initial thrombin-activated phospholipase C-mediated reaction is quinacrine insensitive and is followed by quinacrine-sensitive phospholipase A2 activities. The phosphatidic acid released probably results in cellular calcium gating, which, among other actions, might release arachidonic acid by activating phospholipases A2. Indeed, we have detected a phosphatidic acid-specific phospholipase A2 in platelets, which might have an important role in the liberation of arachidonic acid.

MeSH Terms

Arachidonic Acid Arachidonic Acids/blood Blood Platelets/enzymology,metabolism Calcimycin/pharmacology Humans In Vitro Techniques Phosphatidic Acids/blood Phospholipases A/metabolism Phospholipases A2 Quinacrine/pharmacology Stimulation, Chemical Thrombin/pharmacology

Chemicals

Arachidonic Acids Phosphatidic Acids Arachidonic Acid Calcimycin Phospholipases A Phospholipases A2 Thrombin Quinacrine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lapetina E G

Billah M M

References (9)

9 references, click to expand

Stimulation of phosphatidic acid production in platelets precedes the formation of arachidonate and parallels the release of serotonin.
Biochim Biophys Acta. 1979 May 25;573(2):394-402 PMID: 375988
Phospholipase A2 activity specific for phosphatidic acid. A possible mechanism for the production of arachidonic acid in platelets.
J Biol Chem. 1981 Jun 10;256(11):5399-403 PMID: 7240145
Phospholipid metabolism in stimulated human platelets. Changes in phosphatidylinositol, phosphatidic acid, and lysophospholipids.
J Clin Invest. 1980 Aug;66(2):275-83 PMID: 7400315
Diglyceride lipase: a pathway for arachidonate release from human platelets.
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3238-41 PMID: 290999
The phosphatidylinositol cycle and the regulation of arachidonic acid production.
Nature. 1981 Jul 23;292(5821):367-9 PMID: 6265791
Serine esterase inhibitors block stimulus-induced mobilization of arachidonic acid and phosphatidylinositide-specific phospholipase C activity in platelets.
J Biol Chem. 1980 Jul 10;255(13):6024-7 PMID: 7391002
Transfer of arachidonic acid between phospholipids in rat liver microsomes.
Biochem Biophys Res Commun. 1979 Dec 28;91(4):1399-405 PMID: 526311
Phospholipase A2 and phospholipase C activities of platelets. Differential substrate specificity, Ca2+ requirement, pH dependence, and cellular localization.
J Biol Chem. 1980 Nov 10;255 (21):10227-31 PMID: 7430120
The initial action of thrombin on platelets. Conversion of phosphatidylinositol to phosphatidic acid preceding the production of arachidonic acid.
J Biol Chem. 1981 May 25;256(10):5037-40 PMID: 6785276

Article Info

Journal

Agents and actions

Abbr.

Agents Actions

ISSN

0065-4299

DOI

10.1007/BF01978728

Published

1981-12-00

Pages

536-7

Language

English

Region

Switzerland

NLM ID

0213341

Subset

External Links

PubMed source DOI: 10.1007/BF01978728

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The importance of the production of phosphatidic acid for the release of arachidonic acid in stimulated platelets.

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