Home LiteratureArticle Details
PMID: 6796963 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Thyrotropin-releasing hormone exerts rapid nuclear effects to increase production of the primary prolactin mRNA transcript.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 11 ·1981-11-00 ·Pages 6662-6

Potter E, Nicolaisen AK, Ong ES, Evans RM, Rosenfeld MG

Abstract

This report directly documents that a polypeptide hormone can regulate specific gene expression as a consequence of increasing the levels of a primary genomic transcript. The regulation and expression of the prolactin gene was studied in a cell line (GH4) derived from a rat pituitary tumor. These cells respond to addition of the hypothalamic tripeptide, thyrotropin-releasing hormone (TRH; thyroliberin), by elevation of the levels of mature prolactin mRNA and increase in prolactin biosynthesis. The message induction is preceded by, and apparently consequential to, a comparable rapid increase in the nuclear prolactin RNA precursors, including the putative primary transcript.

MeSH Terms
Animals Cell Line Cell Nucleus/drug effects,metabolism Cloning, Molecular DNA, Recombinant/metabolism Genes/drug effects Kinetics Pituitary Neoplasms Prolactin/genetics RNA, Messenger/genetics Rats Thyrotropin-Releasing Hormone/pharmacology Transcription, Genetic/drug effects
Chemicals
DNA, Recombinant RNA, Messenger Thyrotropin-Releasing Hormone Prolactin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Potter E
Nicolaisen A K
Ong E S
Evans R M
Rosenfeld M G
References (35)
35 references, click to expand
  1. Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1816-20 PMID: 266704
  2. Steroid receptors: elements for modulation of eukaryotic transcription.
    Annu Rev Biochem. 1976;45:721-46 PMID: 183602
  3. Pregrowth hormone messenger RNA: glucocorticoid induction and identification in rat pituitary cells.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2357-61 PMID: 267930
  4. Glucocorticoid-stimulated accumulation of mouse mammary tumor virus RNA: increased rate of synthesis of viral RNA.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2879-83 PMID: 197523
  5. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  6. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  7. Thyroid and glucocorticoid hormones synergistically control growth hormone mRNA in cultured GH1 cells.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):45-9 PMID: 272661
  8. Regulation of prolactin and somatotropin mRNAs by thyroliberin.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1294-8 PMID: 418408
  9. Construction and identification by positive hybridization-translation of a bacterial plasmid containing a rat growth hormone structural gene sequence.
    Nucleic Acids Res. 1978 Jun;5(6):2039-53 PMID: 353736
  10. Regulation of gene expression in chick oviduct.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:605-15 PMID: 354851
  11. Nucleosome structure III: the structure and transcriptional activity of the chromatin containing the ovalbumin and globin genes in chick oviduct nuclei.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:779-91 PMID: 277317
  12. Transcription of the chicken ovalbumin and conalbumin gene during early secondary induction with estrogens.
    Hoppe Seylers Z Physiol Chem. 1978 Oct;359(10):1307-13 PMID: 721067
  13. Comparison of primary and secondary stimulation of male rats by estradiol in terms of prolactin synthesis and mRNA accumulation in the pituitary.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):824-8 PMID: 284404
  14. In vivo effects of estrogen on ovine pituitaries: prolactin and growth hormone biosynthesis and messenger ribonucleic acid translation.
    Endocrinology. 1979 Mar;104(3):729-35 PMID: 436732
  15. Effect of estrogen on gene expression in chicken oviduct: evidence for transcriptional control of ovalbumin gene.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1049-53 PMID: 286292
  16. Regulation of prolactin mRNA analyzed using a specific cDNA probe.
    J Biol Chem. 1979 Aug 25;254(16):8023-30 PMID: 468804
  17. Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.
    J Biol Chem. 1979 Sep 25;254(18):9050-8 PMID: 479179
  18. Prolactin-mediated transcriptional and post-transcriptional control of casein gene expression.
    Cell. 1979 Aug;17(4):1013-23 PMID: 487427
  19. The synthesis and processing of a nuclear RNA precursor to rat pregrowth hormone messenger RNA.
    Nucleic Acids Res. 1979 Jul 11;6(9):3133-44 PMID: 493135
  20. Regulation of protein synthesis by polypeptide hormones and cyclic AMP.
    Adv Cyclic Nucleotide Res. 1979;11:205-64 PMID: 40408
  21. Transcription units for mRNA production in eukaryotic cells and their DNA viruses.
    Prog Nucleic Acid Res Mol Biol. 1979;22:327-53 PMID: 523634
  22. Comparison of potential nuclear precursors for prolactin and growth hormone messenger RNA.
    J Biol Chem. 1980 Mar 25;255(6):2243-6 PMID: 6244273
  23. Dopaminergic inhibition of prolactin synthesis and prolactin messenger RNA accumulation in cultured pituitary cells.
    J Biol Chem. 1980 Sep 10;255(17):8092-7 PMID: 6893323
  24. Structure of the rat prolactin gene.
    J Biol Chem. 1980 Sep 25;255(18):8655-62 PMID: 6251061
  25. Hormonal regulation of peptide receptors and target cell responses.
    Nature. 1979 Jul 12;280(5718):109-16 PMID: 233264
  26. Characterization of rat calcitonin mRNA.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4444-8 PMID: 6933496
  27. Genomic organization of rat prolactin and growth hormone genes.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4583-7 PMID: 6254031
  28. Probable nuclear precursors of preprolactin mRNA in rat pituitary cells.
    J Biol Chem. 1981 Mar 25;256(6):2597-600 PMID: 7204367
  29. Glucocorticoid regulation of metallothionein-I mRNA synthesis in cultured mouse cells.
    J Biol Chem. 1981 Mar 25;256(6):2621-4 PMID: 7204370
  30. Altered expression of the calcitonin gene associated with RNA polymorphism.
    Nature. 1981 Mar 5;290(5801):63-5 PMID: 7207587
  31. Thyroid or glucocorticoid hormone induces pre-growth-hormone mRNA and its probable nuclear precursor in rat pituitary cells.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2230-4 PMID: 6941282
  32. Regulation of protein synthesis by cyclic AMP.
    Adv Cyclic Nucleotide Res. 1974;4(0):335-438 PMID: 4153040
  33. Thyrotropin-releasing hormone regulates the number of its own receptors in the GH3 strain of pituitary cells in culture.
    Biochemistry. 1975 Aug 26;14(17):3845-51 PMID: 169886
  34. Thyrotropin-releasing hormone increases prolactin mRNA activity in the cytoplasm of GH-cells as measured by translation in a wheat germ cell-free system.
    Biochem Biophys Res Commun. 1976 Jun 21;70(4):1180-9 PMID: 821486
  35. Cell-free synthesis of fish preproinsulin, and processing by heterologous mammalian microsomal membranes.
    Proc Natl Acad Sci U S A. 1977 May;74(5):2059-63 PMID: 325565
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.11.6662
Published
1981-11-00
Pages
6662-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349109
Subset
IM
Grants
NIADDK NIH HHS · AM18477 · United States
NIADDK NIH HHS · AM26741 · United States
NIGMS NIH HHS · GM26444 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.11.6662 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2