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PMID: 493135 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The synthesis and processing of a nuclear RNA precursor to rat pregrowth hormone messenger RNA.

Nucleic acids research ·Vol. 6 ·No. 9 ·1979-07-11 ·Pages 3133-44

Harpold MM, Dobner PR, Evans R, Bancroft FC, Darnell JE

Abstract

A recombinant DNA plasmid, pBR322-GH1, which contains about 80% of the sequences of rat pregrowth hormone (pGH) mRNA, allowed an analysis of nuclear RNA from GH3 cells for possible precursors of cytoplasmic pGH mRNA. A single 20-22S RNA SPECIES ABOUT 2-3 TIMes larger than pGH mRNA was detected in nuclear RNA from GH3 cells labeled for 5 min. with 3H-uridine. After longer label times a 12S RNA indistinguishable in size from cytoplasmic 12S pGH mRNA became the predominant labeled RNA complementary to the plasmid pBR322-GH1. Both of these nuclear RNA species contained poly (A). Kinetic analysis of the labeling of nuclear and cytoplasmic pGH mRNA sequences showed that the 20S and 12S nuclear RNA molecules were labeled before significant labeling of cytoplasmic pGH mRNA was detected, and also indicated that there is complete conservation of nuclear pGH mRNA sequences in the production of cytoplasmic pGH mRNA. These results indicate that cytoplasmic pGH mRNA is generated by nuclear processing of a larger nuclear RNA molecule.

MeSH Terms
Animals Cell Line Cell Nucleus/metabolism DNA, Recombinant/metabolism Glioma Growth Hormone/biosynthesis Kinetics Nucleic Acid Hybridization Plasmids Protein Precursors/biosynthesis RNA/metabolism RNA, Messenger/biosynthesis Rats
Chemicals
DNA, Recombinant Protein Precursors RNA, Messenger RNA Growth Hormone
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Harpold M M
Dobner P R
Evans R
Bancroft F C
Darnell J E
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45 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.9.3133
Published
1979-07-11
Pages
3133-44
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327921
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.9.3133 PMC Full Text View journal details
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