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PMID: 6790415 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Application of monoclonal anti-HY antibody for human H-Y typing.

Human genetics ·Vol. 57 ·No. 1 ·1981-00-00 ·Pages 64-7

Koo GC, Tada N, Chaganti R, Hammerling U

Abstract

We have successfully produced monoclonal anti- H-Y antibody by fusing NS-1 myeloma cells with splenocytes from C57BL/6 females immunized with syngeneic male splenocytes. We have proved that the antibody is male specific and that it cross reacts with human H-Y. We have further tested 80 normal individuals for H-Y antigen and obtained significant differences between males and females. Therefore, the monoclonal anti- H-Y antibody is useful for clinical typing of human H-Y antigen.

MeSH Terms
Animals Antibodies Antibodies, Anti-Idiotypic Antibodies, Monoclonal Cell Line Cells, Cultured Cytotoxicity, Immunologic Female Fibroblasts/immunology Gonadal Dysgenesis/genetics H-Y Antigen/genetics Humans Hybrid Cells/immunology Karyotyping Male Mice Mice, Inbred C57BL Plasmacytoma Ranidae Rosette Formation Spleen/immunology Turner Syndrome/genetics
Chemicals
Antibodies Antibodies, Anti-Idiotypic Antibodies, Monoclonal H-Y Antigen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Koo G C
Tada N
Chaganti R
Hammerling U
References (19)
19 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00271169
Published
1981-00-00
Pages
64-7
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
Grants
NCI NIH HHS · CA-08748 · United States
NCI NIH HHS · CP-85665 · United States
NICHD NIH HHS · HD-14357 · United States
External Links
PubMed source DOI: 10.1007/BF00271169 View journal details
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