Topographical location of H-Y antigen on mouse spermatozoa by immunoelectronmicroscopy.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 5 ·1973-05-00 ·Pages 1502-5

Koo GC, Stackpole CW, Boyse EA, Hämmerling U, Lardis MP

Abstract

H-Y (male) antigen was visually located on mouse sperm by electron microscopy, by use of the indirect hybrid antibody method and tobacco mosaic virus as the visual marker. Labeling was achieved by centrifugation of the sperm through a discontinuous gradient consisting of alternating layers of immune reagents and wash solutions. Treated sperm were examined topographically by preparation of platinum-carbon replicas. Antigen was located mainly on the acrosomal cap of the sperm head.

MeSH Terms

Animals Antibody Formation Carbon Cattle/immunology Centrifugation, Density Gradient Female Histocompatibility Antigens/isolation & purification Immune Sera Immunoglobulin G Male Mice Mice, Inbred Strains Microscopy, Electron Platinum Rabbits/immunology Skin Transplantation Specific Gravity Spermatozoa/immunology Tobacco Mosaic Virus/immunology Transplantation, Homologous

Chemicals

Histocompatibility Antigens Immune Sera Immunoglobulin G Platinum Carbon

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Koo G C

Stackpole C W

Boyse E A

Hämmerling U

Lardis M P

References (13)

13 references, click to expand

A comparative view of sperm ultrastructure.
Biol Reprod Suppl. 1970;2:90-127 PMID: 12254595
Synthetic activities during spermatogenesis in the mouse RNA and protein.
Exp Cell Res. 1965 Aug;39(1):197-224 PMID: 5318974
Use of hybrid antibody with anti-gamma-G and anti-ferritin specificities in locating cell surface antigens by electron microscopy.
J Exp Med. 1968 Dec 1;128(6):1461-73 PMID: 4972125
The genetics of the mammalian gamete.
Biol Rev Camb Philos Soc. 1970 Feb;45(1):73-119 PMID: 5310031
Detection of H-2 antigens on mouse spermatozoa by the cytotoxicity test.
Nature. 1970 Nov 7;228(5271):570-2 PMID: 5528693
A freeze-etching study of rabbit spermatozoa with particular reference to head structures.
J Ultrastruct Res. 1970 Dec;33(5):598-614 PMID: 4923167
Serological demonstration of H-Y (male) antigen on mouse sperm.
Nature. 1971 Aug 13;232(5311):478-80 PMID: 4937209
Another visual marker of antibody for electron microscopy.
Virology. 1971 Sep;45(3):858-62 PMID: 4108188
Substructure of the mammalian acrosome.
J Ultrastruct Res. 1972 Mar;38(5):591-604 PMID: 5012824
Serologically demonstrable alloantigens of mouse epidermal cells.
J Exp Med. 1972 Apr 1;135(4):938-55 PMID: 4553016
Human sperm head ultrastructure: a freeze-etching study.
J Ultrastruct Res. 1972 Jun;39(5):520-39 PMID: 4556325
Serological detection of a cell-surface antigen specified by the T (brachyury) mutant gene in the house mouse.
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2076-80 PMID: 4506076
On the structure of the XY bivalent in Mus musculus L.
Chromosoma. 1963;13:521-5 PMID: 13947455

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.5.1502

Published

1973-05-00

Pages

1502-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433529

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.5.1502 PMC Full Text View journal details

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Topographical location of H-Y antigen on mouse spermatozoa by immunoelectronmicroscopy.

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