Home LiteratureArticle Details
PMID: 6788894 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human megakaryocytes. II. Expression of platelet proteins in early marrow megakaryocytes.

The Journal of experimental medicine ·Vol. 154 ·No. 1 ·1981-07-01 ·Pages 88-100

Rabellino EM, Levene RB, Leung LL, Nachman RL

Abstract

Analysis of various platelet proteins by immunofluorescence demonstrated that platelet glycoproteins Ib, IIb, and IIIa, as well as plasma factor VIII antigen (factor VIII:AGN), platelet factor 4, and fibronectin are present in the vast majority of morphologically recognizable megakaryocytes. In addition, a small number of lymphoid-like mononuclear marrow cells, representing approximately 1.4--2.9/10(4) marrow cells, was found to express the same platelet proteins. This population of early marrow megakaryocytes is analogous to small acetylcholinesterase-positive rat and mouse marrow cells. Fc receptors for IgG were expressed in all megakaryocytes and megakaryocyte precursors, whereas the Ia antigen was detected only on a proportion of mature megakaryocytes and not on only early or precursor megakaryocytes. Platelet glycoproteins Ib, IIb, and IIIa, as well as factor VIII:AGN, and platelet factor 4 were established as distinct markers for marrow megakaryocytes and may be helpful for identifying megakaryocytic cells as well as for monitoring events of megakaryocyte differentiation.

MeSH Terms
Antigens Blood Platelets/physiology Blood Proteins Bone Marrow Cells Factor VIII/immunology Fluorescent Antibody Technique Humans Megakaryocytes/cytology,physiology Platelet Factor 4 Receptors, Fc/immunology Time Factors
Chemicals
Antigens Blood Proteins Receptors, Fc Platelet Factor 4 Factor VIII
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rabellino E M
Levene R B
Leung L L
Nachman R L
References (35)
35 references, click to expand
  1. "Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin.
    J Immunol. 1966 Dec;97(6):822-7 PMID: 4163007
  2. Localization of mouse isoantigens on the cell surface as revealed by immunofluorescence.
    Immunology. 1967 Oct;13(4):395-403 PMID: 4168095
  3. Cholinesterase as a possible marker for early cells of the megakaryocytic series.
    Blood. 1973 Sep;42(3):413-21 PMID: 4125240
  4. Synthesis of von Willebrand factor by cultured human endothelial cells.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1906-9 PMID: 4209883
  5. Metabolic and functional studies of monocytes in congenital neutropenia.
    Br J Haematol. 1974 Oct;28(2):233-43 PMID: 4611471
  6. The effect of platelet membrane antibodies on aggregation and release.
    Br J Haematol. 1974 Dec;28(4):551-60 PMID: 4477019
  7. Subcellular platelet factor VIII antigen and von Willebrand factor.
    J Exp Med. 1975 May 1;141(5):1101-13 PMID: 1168691
  8. Platelet physiology and abnormalities of platelet function (first of two parts).
    N Engl J Med. 1975 Sep 11;293(11):531-41 PMID: 168489
  9. Interrelationships of surface immunoglobulin and Fc receptors on mouse B lymphocytes.
    J Immunol. 1975 Dec;115(6):1665-71 PMID: 1102608
  10. The pathogenesis of atherosclerosis (first of two parts).
    N Engl J Med. 1976 Aug 12;295(7):369-77 PMID: 819830
  11. Separation of megakaryocytes from mouse bone marrow by density gradient centrifugation.
    Blood. 1976 Jul;48(1):133-8 PMID: 947401
  12. Biology of megakaryocytes.
    Prog Hemost Thromb. 1976;3:211-29 PMID: 781731
  13. Studies with B-cell allo- and hetero-antisera: parallel reactivity and special properties.
    Scand J Immunol. 1976;5(6-7):745-57 PMID: 62389
  14. Studies of the mechanism of the human platelet release reaction induced by immunologic stimuli. III. Relationship between the binding of soluble IgG aggregates to the Fc receptor and cell response in the presence and absence of plasma.
    J Immunol. 1977 Feb;118(2):514-24 PMID: 557068
  15. Platelet membrane defects in Glanzmann's thrombasthenia. Evidence for decreased amounts of two major glycoproteins.
    J Clin Invest. 1977 Sep;60(3):535-45 PMID: 70433
  16. Synthesis of factor VIII antigen by cultured guinea pig megakaryocytes.
    J Clin Invest. 1977 Oct;60(4):914-21 PMID: 70434
  17. Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):4012-6 PMID: 71738
  18. Membrane receptors of mouse leukocytes. II. Sequential expression of membrane receptors and phagocytic capacity during leukocyte differentiation.
    J Exp Med. 1978 Feb 1;147(2):434-45 PMID: 624905
  19. Regulation of proliferation of murine megakaryocyte progenitor cells by cell cycle.
    Blood. 1978 Jul;52(1):163-70 PMID: 656624
  20. Radioimmunoassay of platelet factor 4 and beta-thromboglobulin: development and application to studies of platelet release in relation to fibrinopeptide A generation.
    Br J Haematol. 1978 May;39(1):129-46 PMID: 78721
  21. The ultrastructural localization of factor VIII-antigen in human platelets, megakaryocytes and endothelial cells utilizing a ferritin-labelled antibody.
    Br J Haematol. 1978 Jun;39(2):209-13 PMID: 678473
  22. Inhibition of human erythropoietic colony formation in culture by treatment with Ia antisera.
    J Exp Med. 1978 Aug 1;148(2):613-8 PMID: 702050
  23. Synthesis of actin by cultured guinea pig megakaryocytes. Complex formation with fibrin.
    Biochim Biophys Acta. 1978 Sep 21;543(1):91-105 PMID: 101253
  24. Platelet factor VIII-related antigen: immunofluorescent localization.
    Thromb Res. 1978 Nov;13(5):871-81 PMID: 84410
  25. Human megakaryocytes. I. Characterization of the membrane and cytoplasmic components of isolated marrow megakaryocytes.
    J Exp Med. 1979 Jun 1;149(6):1273-87 PMID: 571893
  26. Structural analysis of human platelet membrane glycoprotein I complex.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2952-4 PMID: 288080
  27. Thrombocytosis-induced suppression of small acetylcholinesterase-positive cells in bone marrow of rats.
    Blood. 1979 Dec;54(6):1338 PMID: 508941
  28. Surface labelling as a tool to determine structure-function relationships of platelet plasma membrane glycoproteins.
    Thromb Haemost. 1980 Feb 29;42(5):1638-51 PMID: 6768157
  29. Shear rate dependent inhibition of platelet adhesion and aggregation on collagenous surfaces by antibodies to human factor VIII/von Willebrand factor.
    Br J Haematol. 1980 Jan;44(1):127-39 PMID: 6769457
  30. Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny.
    Blood. 1980 Aug;56(2):289-301 PMID: 6994839
  31. Isolation and quantitation of the platelet membrane glycoprotein deficient in thrombasthenia using a monoclonal hybridoma antibody.
    J Clin Invest. 1980 Dec;66(6):1311-8 PMID: 6449521
  32. Immunofluorescent identification of human megakaryocyte colonies using an antiplatelet glycoprotein antiserum.
    Blood. 1981 Feb;57(2):277-86 PMID: 7004535
  33. Reed-Sternberg cells in Hodgkin's disease contain fibronectin.
    Blood. 1981 Feb;57(2):339-42 PMID: 7004537
  34. Isolation, purification, and partial characterization of platelet membrane glycoproteins IIb and IIIa.
    J Biol Chem. 1981 Feb 25;256(4):1994-7 PMID: 6450761
  35. Human megakaryocyte stimulation of proliferation of bone marrow fibroblasts.
    Blood. 1981 Apr;57(4):781-7 PMID: 7470627
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.154.1.88
Published
1981-07-01
Pages
88-100
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2186390
Subset
IM
Grants
NCI NIH HHS · CA-00518/01 · United States
NHLBI NIH HHS · HL-06161-01 · United States
NHLBI NIH HHS · HL-18828 · United States
External Links
PubMed source DOI: 10.1084/jem.154.1.88 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2