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PMID: 4209883 Published · ppublish English Journal Article

Synthesis of von Willebrand factor by cultured human endothelial cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 5 ·1974-05-00 ·Pages 1906-9

Jaffe EA, Hoyer LW, Nachman RL

Abstract

Cultured human endothelial cells synthesize and secrete a protein(s) which has Factor VIII antigen but which lacks Factor VIII clot-promoting activity (J. Clin. Invest. 52, 2757-2764, 1973). Von Willebrand factor activity has been identified in medium from cultured human endothelial cells. This activity was demonstrated by the ability to correct the defect in platelet adhesiveness of blood obtained from patients with von Willebrand's disease. This activity also supported ristocetin-induced aggregation of washed normal human platelets. The von Willebrand factor activity from cultured endothelial cells has physicochemical and immunologic properties like those of the von Willebrand factor activity and the Factor VIII antigen present in human plasma and the Factor VIII antigen synthesized by human endothelial cells in vitro. Rabbit antibody to chromatographic fractions containing endothelial cell von Willebrand factor inhibits the platelet retention of normal blood in glass bead columns.

MeSH Terms
Animals Antigen-Antibody Reactions Biological Assay Blood Platelets Cells, Cultured Endothelium/metabolism Factor VIII/analysis,biosynthesis Humans Immunodiffusion Rabbits/immunology
Chemicals
Factor VIII
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jaffe E A
Hoyer L W
Nachman R L
References (21)
21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.5.1906
Published
1974-05-00
Pages
1906-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388351
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.5.1906 PMC Full Text View journal details
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