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PMID: 6781474 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Enzymic determination of branched-chain amino acids and 2-oxoacids in rat tissues. Transfer of 2-oxoacids from skeletal muscle to liver in vivo.

The Biochemical journal ·Vol. 188 ·No. 3 ·1980-06-15 ·Pages 705-13

Livesey G, Lund P

Abstract

1. A procedure is described for the purification of leucine dehydrogenase (EC 1.4.1.9) from Bacillus subtilis. 2. The preparation is suitable for the quantitative assay of branched-chain amino acids and their 2-oxoacid analogues. 3. The content of total branched-chain 2-oxoacids in freeze-clamped liver, kidney, heart or mammary gland of fed rats is less than 5 nmol/g fresh wt. Higher amounts are present in skeletal muscle and arterial blood (25 +/- 4 nmol per g fresh wt., and 33 +/- 6 nmol per ml respectively; means +/- S.D. of 3 and 11 animals respectively). The values are not significantly affected by starvation for 24 h. 4. Arteriovenous difference measurements show that considerable amounts of branched-chain 2-oxoacids are released by skeletal muscle into the circulation and similar amounts are removed by the liver (about 1 mmol/24 h in a 400 g rat).

MeSH Terms

Amino Acid Oxidoreductases/isolation & purification Amino Acids, Branched-Chain/analysis,metabolism Animals Bacillus subtilis/enzymology Female Keto Acids/analysis,metabolism Kinetics Leucine/isolation & purification Leucine Dehydrogenase Liver/metabolism Male Methods Muscles/metabolism Rats Tissue Distribution

Chemicals

Amino Acids, Branched-Chain Keto Acids Amino Acid Oxidoreductases Leucine Dehydrogenase Leucine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Livesey G

Lund P

References (25)

25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1880705

Published

1980-06-15

Pages

705-13

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1161952

Subset

External Links

PubMed source DOI: 10.1042/bj1880705 PMC Full Text View journal details

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Enzymic determination of branched-chain amino acids and 2-oxoacids in rat tissues. Transfer of 2-oxoacids from skeletal muscle to liver in vivo.

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