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PMID: 728077 Published · ppublish English Journal Article

Interference by branched-chain amino acids in the assay of alanine with alanine dehydrogenase.

The Biochemical journal ·Vol. 174 ·No. 3 ·1978-09-15 ·Pages 1079-82

Lund P, Baverel G

Abstract

Commercial preparations of alanine dehydrogenase from Bacillus subtilis are contaminated to varying extents with activity towards branched-chain amino acids. The Km values for these amino acids are of the same order as for L-alanine (about 10(-3)M). The branched-chain amino acid dehydrogenase activity is lost on dialysis for 2--4h against water or 2mM-EDTA.

MeSH Terms
Alanine/analysis Amino Acid Oxidoreductases Amino Acids, Branched-Chain Keto Acids/analysis Kinetics
Chemicals
Amino Acids, Branched-Chain Keto Acids Amino Acid Oxidoreductases Alanine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lund P
Baverel G
References (12)
12 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1741079
Published
1978-09-15
Pages
1079-82
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1186017
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1741079 PMC Full Text View journal details
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