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PMID: 6780599 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.

Journal of clinical microbiology ·Vol. 13 ·No. 1 ·1981-01-00 ·Pages 1-5

Honda T, Taga S, Takeda Y, Miwatani T

Abstract

The Elek test was modified for detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli. A total of 164 strains of E. coli were tested by the modified Elek test, and the results correlated well with those of the Chinese hamster ovary cell assay and passive immune hemolysis. It is concluded that the modified Elek test is a simple and reproducible assay method for identification of E. coli which produce heat-labile enterotoxin, and is suitable for use in clinical laboratories.

MeSH Terms
Bacteriological Techniques Enterotoxins/analysis,biosynthesis Escherichia coli/metabolism Hot Temperature Immunodiffusion
Chemicals
Enterotoxins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Honda T
Taga S
Takeda Y
Miwatani T
References (25)
25 references, click to expand
  1. Neutralization of cholera enterotoxin-induced steroidogenesis by specific antibody.
    J Infect Dis. 1974 Mar;129(3):284-8 PMID: 4593242
  2. Factors influencing heat-labile Escherichia coli enterotoxin activity.
    Infect Immun. 1976 Aug;14(2):383-8 PMID: 9363
  3. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.
    Infect Immun. 1974 Aug;10(2):320-7 PMID: 4368545
  4. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture.
    Infect Immun. 1975 Feb;11(2):334-6 PMID: 1089601
  5. Antigenic difference between polar montrichous and peritrichous flagella of Vibrio parahaemolyticus.
    J Bacteriol. 1974 Nov;120(2):923-8 PMID: 4142031
  6. The recognition of toxicogenic bacterial strains in vitro.
    Br Med J. 1948 Mar 13;1(4549):493-6 PMID: 18904096
  7. Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus.
    Infect Immun. 1976 Oct;14(4):1028-33 PMID: 1033156
  8. Induction of Escherichia coli and Vibrio cholerae enterotoxins by an inhibitor of protein synthesis.
    Infect Immun. 1977 Jan;15(1):132-7 PMID: 319055
  9. Response of the rabbit ileal loop to cell-free products from Escherichia coli enteropathogenic for swine.
    J Infect Dis. 1970 Feb;121(2):182-7 PMID: 4905648
  10. The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.
    J Med Microbiol. 1970 Aug;3(3):387-401 PMID: 4919579
  11. Enterotoxigenic Escherichia coli isolated from patients with severe cholera-like disease.
    J Infect Dis. 1971 Apr;123(4):378-85 PMID: 4938945
  12. Production of vascular permeability factor by enterotoxigenic Escherichia coli isolated from man.
    Infect Immun. 1973 Nov;8(5):725-30 PMID: 4584048
  13. Identification of enterotoxigenic Escherichia coli and serum antitoxin activity by the vascular permeability factor assay.
    Infect Immun. 1973 Nov;8(5):731-5 PMID: 4584049
  14. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture.
    Science. 1974 Jan 25;183(4122):334-6 PMID: 4587905
  15. Cross-reactivity between heat labile enterotoxins of Vibrio cholerae and Escherichia coli in neutralization tests in rabbit ileum and skin.
    Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Dec;81(6):757-62 PMID: 4592974
  16. Direct serological assay for the heat-labile enterotoxin of Escherichia coli, using passive immune hemolysis.
    Infect Immun. 1977 May;16(2):604-9 PMID: 193793
  17. Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin.
    Infect Immun. 1977 Sep;17(3):541-5 PMID: 332626
  18. Immunological cross-reactivity between a heat-labile enterotoxin(s) of Escherichia coli and subunits of Vibrio cholerae enterotoxin.
    Infect Immun. 1978 Sep;21(3):1036-9 PMID: 361573
  19. Demonstration of shared and unique immunological determinants in enterotoxins from Vibrio cholerae and Escherichia coli.
    Infect Immun. 1978 Dec;22(3):709-13 PMID: 83301
  20. Adaptation of the staphylococcal coagglutination technique for detection of heat-labile enterotoxin of Escherichia coli.
    J Clin Microbiol. 1979 Jan;9(1):49-55 PMID: 372214
  21. Passive immune hemolysis for detection of heat-labile enterotoxin produced by Escherichia coli isolated from different sources.
    Infect Immun. 1979 Jun;24(3):606-10 PMID: 381195
  22. Isolation and characterization of homogeneous heat-labile enterotoxins with high specific activity from Escherichia coli cultures.
    Infect Immun. 1979 Jun;24(3):760-9 PMID: 89088
  23. Immunological methods for detection of Kanagawa phenomenon of Vibrio parahaemolyticus.
    J Clin Microbiol. 1980 Jun;11(6):600-3 PMID: 7430330
  24. Value of passive immune hemolysis for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.
    J Clin Microbiol. 1980 Dec;12(6):768-71 PMID: 7031077
  25. Immunological study of the heat-labile enterotoxins of Escherichia coli and Vibrio cholerae.
    Infect Immun. 1974 Mar;9(3):564-70 PMID: 4206029
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.13.1.1-5.1981
Published
1981-01-00
Pages
1-5
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC273710
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.13.1.1-5.1981 PMC Full Text View journal details
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