Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin.

Infection and immunity ·Vol. 17 ·No. 3 ·1977-09-00 ·Pages 541-5

Greenberg HB, Sack DA, Rodriguez W, Sack RB, Wyatt RG, Kalica AR, Horswood RL, Chanock RM, Kapikian AZ

Abstract

The development of a microtiter solid-phase radioimmunoassay for the detection of Vibrio cholerae enterotoxin and heat-labile Escherichia coli enterotoxin is described. The test is based on the immunological similarity between V. cholerae toxin and E. coli heat-labile toxin. The assay is easy to perform, quantitative, and at least as sensitive and specific as the Y-1 adrenal cell system.

MeSH Terms

Adrenal Glands/cytology Animals Binding Sites, Antibody Culture Media Enterotoxins/analysis Escherichia coli/immunology Hot Temperature Humans Ileum Immunologic Techniques Microchemistry Rabbits Radioimmunoassay/methods Vibrio cholerae/immunology

Chemicals

Culture Media Enterotoxins

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Greenberg H B

Sack D A

Rodriguez W

Sack R B

Wyatt R G

Kalica A R

Horswood R L

Chanock R M

Kapikian A Z

References (15)

15 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.17.3.541-545.1977

Published

1977-09-00

Pages

541-5

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC421159

Subset

External Links

PubMed source DOI: 10.1128/iai.17.3.541-545.1977 PMC Full Text View journal details

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Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin.

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