Transformation and transduction of Bacillus subtilis strains with the Bsu R restriction-modification system by means of modified and unmodified DNA of pUB110 plasmid.

Molecular & general genetics : MGG ·Vol. 180 ·No. 1 ·1980-00-00 ·Pages 135-8

Prozorov AA, Belova TS, Surikov NN

Abstract

During transformation of B. subtilis cells with the Bsu R restriction-modification system by means of pUB110 plasmid restriction and modification of the plasmid DNA occurs. The effect of restriction on the transformation frequency is relatively weak, bringing about 20-fold decrease only. When using cells of a modifying recipient, the frequency of AR9 phage-mediated transduction of unmodified plasmid DNA is also relatively little decreased. The frequency of transduction by chromosomal markers, under the same conditions, falls much lower.

MeSH Terms

Bacillus subtilis/genetics Bacteriophages/genetics DNA, Bacterial/genetics DNA, Viral/genetics Genetic Markers Plasmids Transduction, Genetic Transformation, Genetic

Chemicals

DNA, Bacterial DNA, Viral Genetic Markers

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Prozorov A A

Belova T S

Surikov N N

References (7)

7 references, click to expand

Characterization of plasmid transformation in Bacillus subtilis: kinetic properties and the effect of DNA conformation.
Mol Gen Genet. 1979 Jan 2;167(3):251-8 PMID: 105246
Studies on transduction process by SPP1 phage.
J Gen Virol. 1978 Dec;41(3):563-72 PMID: 106091
Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.
J Bacteriol. 1978 Apr;134(1):318-29 PMID: 418061
Restriction of plasmid-mediated transformation in Bacillus subtilis 168.
Mol Gen Genet. 1979 Sep;175(2):235-7 PMID: 117281
Restriction and modification in B. subtilis. Biological aspects.
Mol Gen Genet. 1974;131(3):181-91 PMID: 4215951
Host-controlled modification and restriction in Bacillus subtilis: Bsu 168-system and BsuR-system in B. subtilis 168.
Mol Gen Genet. 1979 Feb 26;170(2):123-7 PMID: 107391
Inactivation of restriction endonuclease bamNx after infection with phage phi NR2.
Nature. 1979 Jan 4;277(5691):64-6 PMID: 233262

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267362

Published

1980-00-00

Pages

135-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267362

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Transformation and transduction of Bacillus subtilis strains with the Bsu R restriction-modification system by means of modified and unmodified DNA of pUB110 plasmid.

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