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PMID: 6758684 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Thiolutin-resistant mutants of Salmonella typhimurium.

Antimicrobial agents and chemotherapy ·Vol. 22 ·No. 4 ·1982-10-00 ·Pages 541-7

Joshi A, Verma M, Chakravorty M

Abstract

Spontaneous mutants of Salmonella typhimurium isolated in our laboratory from thiolutin-containing tryptone agar plates are partially resistant to thiolutin in enriched media. In minimal media, they are not resistant. The mutants are not temperature sensitive but fail to support the development of phage P22 at higher temperatures (40 degrees C). Thiolutin did not interfere with RNA polymerase or nucleotide kinase in in vitro experiments. However, thiolutin did inhibit the rate of incorporation of exogenous uridine into the cellular pool and consequently the acid-precipitable material. It appears that one site of action of thiolutin is at the membrane level.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacterial Proteins/metabolism DNA-Directed RNA Polymerases/metabolism Drug Resistance, Microbial Mutation Pyrrolidinones/pharmacology Salmonella typhimurium/drug effects,growth & development Thymidine Kinase/metabolism Uridine/metabolism Uridine Kinase/metabolism
Chemicals
Anti-Bacterial Agents Bacterial Proteins Pyrrolidinones acetopyrrothine Thymidine Kinase Uridine Kinase DNA-Directed RNA Polymerases Uridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Joshi A
Verma M
Chakravorty M
References (16)
16 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.22.4.541
Published
1982-10-00
Pages
541-7
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC183790
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.22.4.541 PMC Full Text View journal details
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