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PMID: 7050625 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Participation of the host protein(s) in the morphogenesis of bacteriophage P22.

Molecular & general genetics : MGG ·Vol. 186 ·No. 1 ·1982-00-00 ·Pages 44-9

Joshi A, Siddiqui JZ, Verma M, Chakravorty M

Abstract

Spontaneous mutants of S. typhimurium resistant to thiolutin are conditionally non-permissive for phage P22 development (Joshi and Chakravorty 1979). At 40 degree C non-infective phage particles are produced. Phage development in two nonpermissive hosts (18/MC4 and 153/MC4) has been studied in detail. The steps at which the phage morphogenesis is interfered with differ in the two mutants. The electron micrograph of the particles produced in the mutant 18/MC4 reveals the presence of normal-looking particles; these particles contain phage DNA, adsorb to the permissive host but fail to inject their DNA. The particles produced in the mutant 153/MC4 which fail to adsorb to the host are found to be tail fibre-less. These observations indicate the involvement of host protein(s) in phage P22 morphogenesis.

MeSH Terms
Bacterial Proteins/genetics DNA, Viral/genetics Kinetics Microscopy, Electron Mutation Salmonella Phages/genetics,ultrastructure Salmonella typhimurium/genetics Temperature Viral Proteins/genetics
Chemicals
Bacterial Proteins DNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Joshi A
Siddiqui J Z
Verma M
Chakravorty M
References (22)
22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00422910
Published
1982-00-00
Pages
44-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00422910
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