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PMID: 6752885 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence specificity of exonuclease III from E. coli.

Nucleic acids research ·Vol. 10 ·No. 16 ·1982-08-25 ·Pages 4845-59

Linxweiler W, Hörz W

Abstract

The influence of the nucleotide sequence on the digestion of deoxyribonuclease from E. coli, has been investigated. It was found that the rate at which mononucleotides are released varies in a sequence dependent fashion. C-residues are cleaved off rapidly and G-residues slowly while A and T are released at an intermediate rate. Quantitative analyses of digestion experiments with synthetic DNA fragments made it possible to determine rate constants for the cleavage of several dinucleotide bonds by exonuclease III. These values were found to differ by up to a factor of 3. Summation of the differences can lead to appreciable variation in the overall rate of digestion of a DNA strand. The nucleotide specificity of exonuclease III leads to a transient appearance of a series of discrete DNA fragments intermediate in digestion and a stable set of fragments in limit digests, i.e. at the point when all DNA has become single-stranded. This property of exonuclease III needs to be taken into account for the application of the enzyme in the analysis of nucleoprotein complexes.

MeSH Terms
Animals Base Sequence DNA DNA, Satellite DNA, Single-Stranded Escherichia coli/enzymology Exodeoxyribonucleases/metabolism Kinetics Mice Plasmids Substrate Specificity
Chemicals
DNA, Satellite DNA, Single-Stranded DNA Exodeoxyribonucleases exodeoxyribonuclease III
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Linxweiler W
Hörz W
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25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.16.4845
Published
1982-08-25
Pages
4845-59
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320823
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.16.4845 PMC Full Text View journal details
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