Mithramycin and DIPI: a pair of fluorochromes specific for GC-and AT-rich DNA respectively.

Human genetics ·Vol. 36 ·No. 3 ·1977-05-10 ·Pages 299-305

Schnedl W, Breitenbach M, Stranzinger G

Abstract

The AT specificity of the fluorochromes DIPI and DAPI and the GC specificity of mithramycin are evidenced by observations in human, mouse, and bovine chromosomes. DIPI and DAPI produce a pattern similar to Hoechst 33258 in all three species, whereas mithramycin results in a reverse pattern. The AT-rich centromeric heterochromatin in mouse is brilliantly stained by DIPI or DAPI and remains nearly invisible after mithramycin staining. In the GC-rich centromeric heterochromatin of cattle the opposite behavior is observed.

MeSH Terms

Animals Base Sequence Cattle Fluorescent Dyes Heterochromatin Humans In Vitro Techniques Karyotyping Mice Plicamycin Staining and Labeling

Chemicals

Fluorescent Dyes Heterochromatin Plicamycin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Schnedl W

Breitenbach M

Stranzinger G

References (29)

29 references, click to expand

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Article Info

Journal

Human genetics

Abbr.

Hum Genet

ISSN

0340-6717

DOI

10.1007/BF00446280

Published

1977-05-10

Pages

299-305

Language

English

Region

Germany

NLM ID

7613873

Subset

External Links

PubMed source DOI: 10.1007/BF00446280 View journal details

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Mithramycin and DIPI: a pair of fluorochromes specific for GC-and AT-rich DNA respectively.

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