Home LiteratureArticle Details
PMID: 67012 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Optical Studies of the interaction of 4'-6'-diamidino-2-phenylindole with DNA and metaphase chromosomes.

Chromosoma ·Vol. 60 ·No. 1 ·1977-03-07 ·Pages 15-25

Lin MS, Comings DE, Alfi OS

Abstract

The optical absorption and fluorescence characteristics of 4'-6-diamidino-2-phenylindole (DAPI) with DNA and chromosomes were studied. There is a decrease in extinction coefficient and chift in the absorption spectra to a higher wavelength when the dye binds to DNA. The fluorescence of DAPI is enhanced by both A-T and G-C base-pairs. The enhancement by A-T rich is significantly greater than by G-C rich DNA. The chromosomes and the constrictions of human chromosomes 1 and 16; these regions are known to contain A-T rich DNA and show dull fluorescence when treated with quinacrine. This dye may be useful for identifying A-T rich region in chromosomes. The fluorescence of DAPI bound to polynucleotides or chromosomes is partially quenched by the introduction of BrdU. This suppression of dye fluorescence allows optical detection of sister chromatid exchanges and chromosome region containing DNA with an unequal distribution of thymidine between polynucleotide chains after BrdU incorporation.

MeSH Terms
Adenine Nucleotides/analysis Amidines Animals Binding Sites Bromodeoxyuridine Chromosomes/analysis DNA/analysis Fluorescent Dyes Humans Hydrogen-Ion Concentration Indoles Mice Mitosis Polynucleotides/analysis Spectrometry, Fluorescence Staining and Labeling Thymine Nucleotides/analysis
Chemicals
Adenine Nucleotides Amidines Fluorescent Dyes Indoles Polynucleotides Thymine Nucleotides DAPI DNA Bromodeoxyuridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lin M S
Comings D E
Alfi O S
References (22)
22 references, click to expand
  1. Differential fluorescence of sister chromatids with 4'-6-diamidino-2-phenylindole.
    Can J Genet Cytol. 1976 Sep;18(3):545-7 PMID: 63310
  2. Chromosomal localization of mouse satellite DNA.
    Science. 1970 Jun 12;168(3937):1356-8 PMID: 5462793
  3. Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3395-9 PMID: 4128545
  4. Microfluorometric analysis of deoxyribonucleic acid replication kinetics and sister chromatid exchanges in human chromosomes.
    J Histochem Cytochem. 1974 Jul;22(7):478-91 PMID: 4137061
  5. Chromosome regions containing DNAs of known base composition, specifically evidenced by 2,7-di-t-butyl proflavine. Comparison with the Q-banding and relation to dye-DNA interactions.
    Chromosoma. 1974;47(3):263-81 PMID: 4139005
  6. Mechanisms of chromosome banding. IX. Are variations in DNA base composition adequate to account for quinacrine, Hoechst 33258 and daunomycin banding?
    Chromosoma. 1976 Jul 8;56(3):199-211 PMID: 61106
  7. A non-intercalating proflavine derivative.
    Eur J Biochem. 1973 Nov 1;39(1):223-34 PMID: 4770794
  8. Spin-orbital probes of biomolecular structure. A model DNA-acridine system.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2198-202 PMID: 4340750
  9. Microfluorometric detection of asymmetry in the centromeric region of mouse chromosomes.
    Exp Cell Res. 1974 Jun;86(2):392-5 PMID: 4842349
  10. Relationship of centromeric heterochromatin to fluorescent banding patterns of metaphase chromosomes in the mouse.
    Nature. 1971 Jun 25;231(5304):503-6 PMID: 4103792
  11. Detection of sister chromatid exchanges by 4'-6-diamidino-2-phenylindole fluorescence.
    Chromosoma. 1976 Sep 10;57(3):219-25 PMID: 63357
  12. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses.
    Nature. 1975 Feb 6;253(5491):461-2 PMID: 46112
  13. Staining of constitutive heterochromatin in mammalian chromosomes with a new fluorochrome.
    Exp Cell Res. 1972 Nov;75(1):122-6 PMID: 4117919
  14. Apparent dispersive replication of yeast mitochondrial DNA as revealed by density labelling experiments.
    Mol Gen Genet. 1974;131(3):193-207 PMID: 4612326
  15. Chemical differentiation along metaphase chromosomes.
    Exp Cell Res. 1968 Jan;49(1):219-22 PMID: 5640698
  16. Optical studies of the interaction of 33258 Hoechst with DNA, chromatin, and metaphase chromosomes.
    Chromosoma. 1975 Nov 11;52(4):297-316 PMID: 1192901
  17. Mechanisms of chromosome banding. VIII. Hoechst 33258-DNA interaction.
    Chromosoma. 1975 Oct 14;52(3):229-43 PMID: 1183300
  18. Mechanisms of chromosome banding. V. Quinacrine banding.
    Chromosoma. 1975;50(2):111-4 PMID: 48455
  19. Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA.
    Chromosoma. 1974;46(3):255-60 PMID: 4136742
  20. The isolation of complementary strands from a mouse DNA fraction.
    Proc Natl Acad Sci U S A. 1967 Jun;57(6):1729-34 PMID: 5231407
  21. Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythymidylaterich regions in DNA.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):629-32 PMID: 4111050
  22. Location of satellite and homogeneous DNA sequences on human chromosomes.
    Nat New Biol. 1971 Oct 27;233(43):268-71 PMID: 4107945
Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00330407
Published
1977-03-07
Pages
15-25
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330407 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2