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PMID: 63310 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Differential fluorescence of sister chromatids with 4'-6-diamidino-2-phenylindole.

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie ·Vol. 18 ·No. 3 ·1976-09-00 ·Pages 545-7

Lin MS, Alfi OS, Donnell GN

Abstract

Differential fluorescence of sister chromatids and sister chromatid exchanges (SCE) in chromosomes from human lymphocytes grown two replication cycles in medium containing 5-bromodeoxyuridine can be detected by fluorescence microscopy after staining with 4'-6-diamidino-2-phenylindole (DAPI). The DAPI fluorescence appears to be more stable than that of the dye 33258 Hoechst and may provide a more sensitive method for the detection of SCE.

MeSH Terms

Chromatids/physiology Crossing Over, Genetic Fluorescence Fluorescent Dyes Humans Leukocytes/ultrastructure Staining and Labeling

Chemicals

Fluorescent Dyes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lin M S

Alfi O S

Donnell G N

Article Info

Journal

Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie

Abbr.

Can J Genet Cytol

ISSN

0008-4093

DOI

10.1139/g76-068

Published

1976-09-00

Pages

545-7

Language

English

Region

Canada

NLM ID

0372706

Subset

External Links

PubMed source DOI: 10.1139/g76-068

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Differential fluorescence of sister chromatids with 4'-6-diamidino-2-phenylindole.

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