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PMID: 6682114 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mitotic architecture of the cell: the filament networks of the nucleus and cytoplasm.

The Journal of cell biology ·Vol. 96 ·No. 3 ·1983-03-00 ·Pages 896-906

Capco DG, Penman S

Abstract

The skeletal framework of cells at the various stages of mitosis are prepared by extraction with nonionic detergent and examined by stereoscopic whole mount electron microscopy. The insoluble filament network remaining after the detergent-extraction and the depolymerization of microtubules is shown. The nonchromatin filament network of the nucleus, or nuclear matrix, becomes visible as the chromatin condenses at prophase. Filaments are associated with the chromosomes throughout mitosis. Parts of the chromosomes are associated with or are near the nuclear lamina at early stages. The nuclear lamina disappears at metaphase while chromosomes remain associated with filaments now continuous with the cytoplasmic network. Microtubules appear to be unnecessary for maintaining the chromosome position in these preparations since comparison of cells with and without microtubules shows no gross change in chromosome arrangement. The cellular filament network at metaphase and anaphase appears continuous from the plasma lamina to the chromosomes. The filament networks visualized here may be responsible for the prometaphase chromosome movement and participate in the formation of the midbody.

MeSH Terms
Anaphase Animals Cell Line Cell Nucleus/ultrastructure Chromosomes/physiology,ultrastructure Cytoskeleton/ultrastructure Interphase Macropodidae Metaphase Microscopy, Electron Mitosis Nuclear Envelope/ultrastructure Prophase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Capco D G
Penman S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.96.3.896
Published
1983-03-00
Pages
896-906
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112394
Subset
IM
Grants
NCI NIH HHS · CA 08416 · United States
NCI NIH HHS · CA 12174 · United States
NCRR NIH HHS · RR 01219 · United States
External Links
PubMed source DOI: 10.1083/jcb.96.3.896 PMC Full Text View journal details
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