Breakdown and assembly of polyribosomes in synchronized Chinese hamster cells.

Science (New York, N.Y.) ·Vol. 161 ·No. 3843 ·1968-08-23 ·Pages 791-3

Steward DL, Shaeffer JR, Humphrey RM

Abstract

Polyribosomes were absent in metaphase Chinese hamster cells and were reassembled after mitosis. This assembly correlated temporally with formation of nuclear membrane and decondensation of chromatin, accounted for a threefold increased rate of protein synthesis, and occurred independently of de novo RNA synthesis, that is, it utilized preexisting messenger RNA.

MeSH Terms

Amino Acids/metabolism Animals Carbon Isotopes Centrifugation, Density Gradient Cricetinae Culture Techniques Dactinomycin/pharmacology Depression, Chemical Mitosis Protein Biosynthesis RNA/biosynthesis RNA, Messenger/metabolism Ribosomes/metabolism Tritium Uridine/metabolism

Chemicals

Amino Acids Carbon Isotopes RNA, Messenger Tritium Dactinomycin RNA Uridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Steward D L

Shaeffer J R

Humphrey R M

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.161.3843.791

Published

1968-08-23

Pages

791-3

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.161.3843.791 View journal details

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Breakdown and assembly of polyribosomes in synchronized Chinese hamster cells.

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