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PMID: 666747 Published · ppublish English Journal Article

Calcium-dependent Golgi-vesicle fusion and cathepsin B in the conversion of proalbumin into albumin in rat liver.

The Biochemical journal ·Vol. 172 ·No. 2 ·1978-05-15 ·Pages 301-9

Quinn PS, Judah JD

Abstract

1. An enzyme from rat liver that converts proalbumin into albumin is described. Partial purification, inhibitor studies and the conditions for maximum activity suggest that the enzyme is cathepsin B. 2. A membrane-bound enzyme, located mainly in lysosomes, also converts proalbumin into albumin. This appears to be a membrane-bound form of cathepsin B. 3. Isolated Golgi vesicles, incubated under conditions suitable for cathepsin B, convert endogenous proalbumin into albumin. 4. This conversion in Golgi vesicles has an absolute requirement for Ca2+ at micromolar concentrations. Mg2+ does not affect or substitute for Ca2+. Both the proalbumin and the albumin formed from it are intravesicular. 5. Converting activity is enhanced by pretreatment with the known chemical fusogen, poly(ethyleneglycol). 6. Vesicles preincubated at pH above 7 in the presence of dithiothreitol show a marked fall in converting activity. This can be partially restored by incubation with native vesicles. These results suggest that vesicle fusion is a requirement for conversion of proalbumin into albumin.

MeSH Terms
Animals Calcium/pharmacology Cathepsins/metabolism Dithiothreitol/pharmacology Golgi Apparatus/drug effects,metabolism In Vitro Techniques Liver/drug effects,enzymology,metabolism Polyethylene Glycols/pharmacology Prealbumin/metabolism Rats Serum Albumin/biosynthesis,metabolism
Chemicals
Prealbumin Serum Albumin Polyethylene Glycols Cathepsins Calcium Dithiothreitol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Quinn P S
Judah J D
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22 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1720301
Published
1978-05-15
Pages
301-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1185696
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1720301 PMC Full Text View journal details
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