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PMID: 6624880 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Enhancement of human polymorphonuclear leukocyte adherence by the phospholipid mediator 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphorylcholine (AGEPC).

The American journal of pathology ·Vol. 113 ·No. 1 ·1983-10-00 ·Pages 85-9

Valone FH, Goetzl EJ

Abstract

The phospholipid mediator 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphorylcholine (AGEPC) increases human polymorphonuclear leukocyte (PMN) adherence to Sephadex G-25 in manner directly proportional to the concentration of AGEPC from 2 to 650 nM. The maximal enhancement of PMN adherence of 85% +/- 23% (mean +/- SD) evoked by 650 nM AGEPC was indistinguishable in magnitude from that elicited by an optimal concentration of the chemotactic peptide N-f-Met-Leu-Phe. The results of analyses of the relative PMN adherence-enhancing activities of analogs of AGEPC suggest distinct molecular requirements for the enhancement of adherence. PMN adherence increases within 1 minute after exposure of the leukocytes to AGEPC, and the augmented adherence persists for over 30 minutes in spite of extensive washing of the leukocytes. Indomethacin partially blocked enhancement of adherence by AGEPC, suggesting that thromboxane A2 mediates in part the enhancement of adherence by AGEPC.

MeSH Terms
Adult Cell Adhesion/drug effects Dose-Response Relationship, Drug Humans Indomethacin/pharmacology N-Formylmethionine Leucyl-Phenylalanine/pharmacology Neutrophils/physiology Platelet Activating Factor/physiology Thromboxane A2/metabolism
Chemicals
Platelet Activating Factor Thromboxane A2 N-Formylmethionine Leucyl-Phenylalanine Indomethacin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Valone F H
Goetzl E J
References (16)
16 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1983-10-00
Pages
85-9
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1916290
Subset
IM
Grants
PHS HHS · A1-18262 · United States
External Links
PubMed source PMC Full Text View journal details
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