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PMID: 6587322 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Elimination of mRNA splicing by a point mutation outside the conserved GU at 5' splice sites.

Nucleic acids research ·Vol. 12 ·No. 9 ·1984-05-11 ·Pages 3821-7

Montell C, Berk AJ

Abstract

Nearly all mRNA introns begin with the dinucleotide GU. Mutations in either of these virtually invariant bases have been found to inactivate the corresponding 5' splice site. Until now single base changes in neighboring bases have not been found to completely inactivate a 5' splice site. Here we show that a single A----U transversion in the third position of the adenovirus 2 E1A 13S mRNA intron does prevent RNA splicing at the corresponding 5' splice site.

MeSH Terms

Adenoviruses, Human/genetics Base Sequence Coliphages/genetics Dinucleoside Phosphates HeLa Cells/metabolism Humans Mutation Nucleic Acid Hybridization Oligonucleotides/analysis RNA Splicing RNA, Messenger/genetics

Chemicals

Dinucleoside Phosphates Oligonucleotides RNA, Messenger

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Montell C

Berk A J

References (31)

31 references, click to expand

The primary structure of the duck alpha D-globin gene: an unusual 5' splice junction sequence.
EMBO J. 1983;2(8):1339-43 PMID: 10872328
Complete transformation by adenovirus 2 requires both E1A proteins.
Cell. 1984 Apr;36(4):951-61 PMID: 6705049
A new method for sequencing DNA.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
J Gen Virol. 1977 Jul;36(1):59-74 PMID: 886304
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
Cell. 1977 Nov;12(3):721-32 PMID: 922889
Mutagenesis at a specific position in a DNA sequence.
J Biol Chem. 1978 Sep 25;253(18):6551-60 PMID: 681366
Structure of the adenovirus 2 early mRNAs.
Cell. 1978 Jul;14(3):695-711 PMID: 688389
Efficient correction of a mutation by use of chemically synthesized DNA.
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4268-70 PMID: 279913
Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4853-7 PMID: 283395
Adenovirus 5 DNA sequences present and RNA sequences transcribed in transformed human embryo kidney cells (HEK-Ad-5 or 293).
Virology. 1979 Apr 30;94(2):460-9 PMID: 452423
Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
Cell. 1979 Aug;17(4):935-44 PMID: 487437
Are snRNPs involved in splicing?
Nature. 1980 Jan 10;283(5743):220-4 PMID: 7350545
The structure of adenovirus 2 early nuclear and cytoplasmic RNAs.
J Mol Biol. 1980 Feb 15;137(1):23-48 PMID: 7365795
A mechanism for RNA splicing.
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1877-9 PMID: 6246511
Transforming proteins of human adenovirus 5: studies with infected and transformed cells.
Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:477-91 PMID: 6933044
Correlation between splicing sites within an intron and their sequence complementarity with U1 RNA.
Cell. 1980 Oct;21(3):689-96 PMID: 6159982
Adenovirus terminal protein protects single stranded DNA from digestion by a cellular exonuclease.
Nucleic Acids Res. 1980 Feb 11;8(3):543-54 PMID: 6255444
An adenovirus mutant defective in splicing RNA from early region 1A.
Nature. 1981 Jun 11;291(5815):508-10 PMID: 6262659
Novel models for RNA splicing that involve a small nuclear RNA.
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4471-4 PMID: 6170064
Expression of early adenovirus genes requires a viral encoded acidic polypeptide.
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6121-5 PMID: 6947217
Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site.
Nature. 1982 Feb 4;295(5848):380-4 PMID: 7057903
A catalogue of splice junction sequences.
Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
The TATA homology and the mRNA 5' untranslated sequence are not required for expression of essential adenovirus E1A functions.
Cell. 1982 May;29(1):139-48 PMID: 7105179
Nucleotide sequences from the adenovirus-2 genome.
J Biol Chem. 1982 Nov 25;257(22):13475-91 PMID: 7142161
A single-base change at a splice site in a beta 0-thalassemic gene causes abnormal RNA splicing.
Cell. 1982 Jul;29(3):903-11 PMID: 7151176
Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit beta-globin gene.
Nature. 1983 Jan 6;301(5895):38-43 PMID: 6296682
Alternative RNA splicing of the murine alpha A-crystallin gene: protein-coding information within an intron.
Cell. 1983 Mar;32(3):707-12 PMID: 6187470
The nucleotide sequence of the adult chicken alpha-globin genes.
J Biol Chem. 1983 Apr 10;258(7):4623-9 PMID: 6300093
Specific transcription and RNA splicing defects in five cloned beta-thalassaemia genes.
Nature. 1983 Apr 14;302(5909):591-6 PMID: 6188062
Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA.
Nature. 1983 Oct 13-19;305(5935):600-5 PMID: 6194440
Host-range mutants of adenovirus type 5 defective for growth in HeLa cells.
Virology. 1977 Mar;77(1):319-29 PMID: 841862

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.9.3821

Published

1984-05-11

Pages

3821-7

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318792

Subset

Grants

NIAID NIH HHS · AI 07116 · United States

NCI NIH HHS · CA 25235 · United States

NIGMS NIH HHS · GM 07104 · United States

External Links

PubMed source DOI: 10.1093/nar/12.9.3821 PMC Full Text View journal details

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Elimination of mRNA splicing by a point mutation outside the conserved GU at 5' splice sites.

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