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PMID: 6584901 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Biogenesis of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, an integral glycoprotein of the endoplasmic reticulum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 6 ·1984-03-00 ·Pages 1674-8

Brown DA, Simoni RD

Abstract

Using a cell line, C100, that overproduces 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase; EC 1.1.1.34) 100-fold, we have studied the synthesis and insertion of this protein into the endoplasmic reticulum. The enzyme is synthesized on membrane-bound polysomes. It is cotranslationally but not post-translationally inserted into dog pancreatic microsomes. This cotranslational insertion is dependent upon signal recognition particle. HMG-CoA reductase is glycosylated with an oligosaccharide(s) of the "high-mannose" type sensitive to endo-beta-D-N-acetylglucosaminidase H. Partial determination of the NH2-terminal amino acid sequence of the in vitro translation product and the mature polypeptide indicate they are the same and demonstrate there is no cleavage of an NH2-terminal signal sequence.

MeSH Terms
Amino Acid Sequence Animals Cell Line Cricetinae Dogs Endoplasmic Reticulum/metabolism Glycoproteins/biosynthesis Hydroxymethylglutaryl CoA Reductases/biosynthesis Intracellular Membranes/metabolism Membrane Proteins/biosynthesis Peptides/metabolism Polyribosomes/metabolism Protein Biosynthesis Protein Precursors/metabolism Protein Sorting Signals
Chemicals
Glycoproteins Membrane Proteins Peptides Protein Precursors Protein Sorting Signals Hydroxymethylglutaryl CoA Reductases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown D A
Simoni R D
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.6.1674
Published
1984-03-00
Pages
1674-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344980
Subset
IM
Grants
NIGMS NIH HHS · GM07276 · United States
NHLBI NIH HHS · HL26502 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.6.1674 PMC Full Text View journal details
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