Replication of an enteric bovine coronavirus in intestinal organ cultures.

Archives of virology ·Vol. 57 ·No. 1 ·1978-00-00 ·Pages 43-51

Bridger JC, Caul EO, Egglestone SI

Abstract

A coronavirus isolated in tracheal organ cultures from the faeces of a calf with diarrhoaea readily multiplied on passage in intestinal organ cultures. Evidence for multiplication was obtained by the production of viral haemagglutinin in organ culture fluids and the presence of immunofluorescence and viral particles in the columnar epithelial cells of the villi. Thus virus multiplication was studied in vitro in the cell type in which it multiplies naturally.

MeSH Terms

Animals Cattle Coronaviridae/growth & development,immunology,isolation & purification Diarrhea/microbiology Feces/microbiology Hemagglutinins, Viral/analysis Intestine, Small Organ Culture Techniques Virus Replication

Chemicals

Hemagglutinins, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bridger J C

Caul E O

Egglestone S I

References (10)

10 references, click to expand

Growth of viruses in organ cultures of intestine.
Br J Exp Pathol. 1970 Apr;51(2):210-6 PMID: 4316099
Coronavirus propagated from patient with non-bacterial gastroenteritis.
Lancet. 1975 Nov 15;2(7942):953-4 PMID: 53434
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J Infect Dis. 1970 Sep;122(3):227-31 PMID: 4317758
Viral enteritis of calves.
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In vitro cultivation in human fetal intestinal organ culture of a reovirus-like agent associated with nonbacterial gastroenteritis in infants and children.
J Infect Dis. 1974 Nov;130(5):523-8 PMID: 4608429
Pattern of shedding of the Norwalk particle in stools during experimentally induced gastroenteritis in volunteers as determined by immune electron microscopy.
J Infect Dis. 1975 Jul;132(1):28-34 PMID: 1151121
Letter: Probable in-vitro cultivation of human reovirus-like agent of infantile diarroea.
Lancet. 1976 Jan 10;1(7950):98-9 PMID: 54627
Growth of porcine transmissible gastroenteritis (TGE) virus in organ cultures of pig tissue.
Nature. 1970 Sep 26;227(5265):1348-9 PMID: 4989435
Further studies on human enteric coronaviruses.
Arch Virol. 1977;54(1-2):107-17 PMID: 196571
Neonatal calf diarrhea: propagation, attenuation, and characteristics of a coronavirus-like agent.
Am J Vet Res. 1973 Feb;34(2):145-50 PMID: 4568246

Article Info

Journal

Archives of virology

Abbr.

Arch Virol

ISSN

0304-8608

DOI

10.1007/BF01315636

Published

1978-00-00

Pages

43-51

Language

English

Region

Austria

NLM ID

7506870

PMCID

PMC7087085

Subset

External Links

PubMed source DOI: 10.1007/BF01315636 PMC Full Text View journal details

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Replication of an enteric bovine coronavirus in intestinal organ cultures.

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