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PMID: 6541126 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tumor promoter PMA stimulates the synthesis and secretion of mouse pro-urokinase in MSV-transformed 3T3 cells: this is mediated by an increase in urokinase mRNA content.

The EMBO journal ·Vol. 3 ·No. 8 ·1984-08-00 ·Pages 1901-6

Belin D, Godeau F, Vassalli JD

Abstract

In mouse MSV-3T3 cells the synthesis of the urokinase form of plasminogen activator was increased 10-fold after addition of the tumor promoter phorbol-12-myristate-13-acetate (PMA). PMA also stimulated the secretion of the protein into the culture medium, mostly in the form of enzymatically inactive pro-urokinase. When assayed by injecting RNA into Xenopus laevis oocytes, the concentration of functional urokinase mRNA was found to be 6- to 10-fold higher in the PMA-treated cells; a similar increase in urokinase mRNA content was measured by hybridisation with a mouse urokinase cDNA probe. Thus, the induction of plasminogen activator by PMA in MSV-3T3 cells is accounted for by an increased content of urokinase mRNA.

MeSH Terms
Animals Carcinogens/pharmacology Cell Line Cell Transformation, Viral Enzyme Precursors/biosynthesis Female Fibroblasts/metabolism Gene Expression Regulation/drug effects Mice Oocytes/metabolism Phorbols/pharmacology RNA, Messenger/biosynthesis Tetradecanoylphorbol Acetate/pharmacology Urokinase-Type Plasminogen Activator/biosynthesis,genetics,metabolism Xenopus laevis
Chemicals
Carcinogens Enzyme Precursors Phorbols RNA, Messenger Urokinase-Type Plasminogen Activator Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Belin D
Godeau F
Vassalli J D
References (40)
40 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Plasminogen: purification from human plasma by affinity chromatography.
    Science. 1970 Dec 4;170(3962):1095-6 PMID: 5475635
  3. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  4. Tumour promotor induces plasminogen activator.
    Nature. 1976 Jan 22;259(5540):232-3 PMID: 1250353
  5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  6. Macrophage plasminogen activator: induction by concanavalin A and phorbol myristate acetate.
    Cell. 1977 Jul;11(3):695-705 PMID: 884740
  7. Secretion of plasminogen activator by human polymorphonuclear leukocytes. Modulation by glucocorticoids and other effectors.
    J Exp Med. 1977 Dec 1;146(6):1693-706 PMID: 200699
  8. Effects of acidified fetal bovine serum on the fibrinolytic activity and growth of cells in culture.
    J Cell Physiol. 1978 Sep;96(3):361-9 PMID: 27528
  9. A study of proteases and protease-inhibitor complexes in biological fluids.
    J Exp Med. 1978 Jul 1;148(1):223-34 PMID: 78958
  10. Specificity of response in hamster cells induced to produce plasminogen activator by the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate.
    Cancer Res. 1978 Nov;38(11 Pt 1):3854-60 PMID: 81105
  11. Immunoprecipitation of specific polysomes using Staphylococcus aureus: purification of the immunoglobulin k chain messenger RNA from the mouse myeloma MPC11.
    Biochemistry. 1978 Dec 12;17(25):5560-6 PMID: 103574
  12. Plasminogen activator from cells transformed by an oncogenic virus: inhibitors of the activation reaction.
    Biochim Biophys Acta. 1979 Jan 12;566(1):138-51 PMID: 215232
  13. Modulation and mapping of a human plasminogen activator by cell fusion.
    Cell. 1978 Dec;15(4):1331-40 PMID: 569557
  14. Inhibition of a plasminogen activator from oncogenic virus-transformed mouse cells by rabbit antibodies against the enzyme.
    Biochim Biophys Acta. 1980 Jun 5;630(1):146-51 PMID: 6248125
  15. Experimental model for quantitative study of metastasis.
    Cancer Res. 1980 Jul;40(7):2300-9 PMID: 7190062
  16. Protease-nexin: a cellular component that links thrombin and plasminogen activator and mediates their binding to cells.
    Cell. 1980 Aug;21(1):37-45 PMID: 6157479
  17. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  18. Purification and characterization of a plasminogen activator from mouse cells transformed by an oncogenic virus.
    Biochim Biophys Acta. 1980 Jun 13;613(2):542-55 PMID: 6256003
  19. Tumor-promoting phorbol esters affect production of prolactin and growth hormone by rat pituitary cells.
    Endocrinology. 1981 Apr;108(4):1164-70 PMID: 7472264
  20. Purification and characterization of the plasminogen activator secreted by human melanoma cells in culture.
    J Biol Chem. 1981 Jul 10;256(13):7035-41 PMID: 6787058
  21. Microinjected Xenopus oocytes synthesize active human plasminogen activator.
    Nucleic Acids Res. 1981 Jul 24;9(14):3355-63 PMID: 6169005
  22. Two independent aspects of tumor promotion.
    Biochim Biophys Acta. 1981 Aug 31;651(1):51-63 PMID: 7025906
  23. Effects of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate on newly synthesized proteins in mouse epidermis.
    Cell. 1981 Aug;25(2):441-9 PMID: 6169440
  24. Regulation of plasminogen activator in 3T3 cells: effect of phorbol myristate acetate on subcellular distribution and molecular weight.
    J Cell Biol. 1981 Sep;90(3):727-31 PMID: 7197280
  25. Regulation of gene expression by tumor promoters. III. Complementation of the developmental program in myeloid leukemic cells by regulating mRNA production and mRNA translation.
    Int J Cancer. 1981 Nov 15;28(5):615-20 PMID: 6975761
  26. The production of distinct forms of plasminogen activator by mouse embryonic cells.
    Dev Biol. 1982 Mar;90(1):154-9 PMID: 6174380
  27. Tumor promoters increase the synthesis of a 32,000-dalton protein in BALB/c 3T3 cells.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1800-4 PMID: 6952230
  28. A proenzyme form of human urokinase.
    J Biol Chem. 1982 Jun 25;257(12):7262-8 PMID: 6806270
  29. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
    J Biol Chem. 1982 Jul 10;257(13):7847-51 PMID: 7085651
  30. Plasminogen activator released as inactive proenzyme from murine cells transformed by sarcoma virus.
    Eur J Biochem. 1982 May 17;124(2):409-14 PMID: 6284507
  31. Effects of cholera toxin and actinomycin on synthesis of [35s]methionine-labeled proteins during progesterone-induced maturation of Xenopus laevis oocytes.
    Dev Biol. 1982 May;91(1):36-42 PMID: 7095262
  32. The primary structure of high molecular mass urokinase from human urine. The complete amino acid sequence of the A chain.
    Hoppe Seylers Z Physiol Chem. 1982 Oct;363(10):1155-65 PMID: 6754569
  33. The complete amino acid sequence of low molecular mass urokinase from human urine.
    Hoppe Seylers Z Physiol Chem. 1982 Sep;363(9):1043-58 PMID: 6754572
  34. Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli.
    Nature. 1983 Jan 20;301(5897):214-21 PMID: 6337343
  35. Hormonal regulation of plasminogen activator mRNA production in porcine kidney cells.
    Cell. 1983 Apr;32(4):1181-90 PMID: 6687701
  36. Effects of 12-O-tetradecanoylphorbol 13-acetate on the production of mRNAs for human tissue-type plasminogen activator.
    Eur J Biochem. 1983 Apr 5;131(3):481-7 PMID: 6682377
  37. Phorbol ester and mitogens stimulate human fibroblast secretions of plasmin-activatable plasminogen activator and protease nexin, an antiactivator/antiplasmin.
    J Cell Biol. 1983 Aug;97(2):323-8 PMID: 6224800
  38. Plasminogen activator secretion of human tumors in short-term organ culture, including a comparison of primary and metastatic colon tumors.
    Cancer Res. 1983 Nov;43(11):5517-25 PMID: 6193874
  39. Complex-formation and inhibition of urokinase by blood plasma proteins.
    Biochem J. 1983 Oct 1;215(1):123-31 PMID: 6194790
  40. Antibodies to plasminogen activator inhibit human tumor metastasis.
    Cell. 1983 Dec;35(3 Pt 2):611-9 PMID: 6418388
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-08-00
Pages
1901-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557615
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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