Home LiteratureArticle Details
PMID: 6501428 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Lateral diffusion of wild-type and mutant Ld antigens in L cells.

The Journal of cell biology ·Vol. 99 ·No. 6 ·1984-12-00 ·Pages 2333-5

Edidin M, Zuniga M

Abstract

We have compared the lateral diffusion of intact transmembrane proteins, wild-type H-2Ld antigens, with that of mutants truncated in the cytoplasmic domain. Diffusion coefficients and mobile fractions were similar for all molecules examined, from wild-type Ld antigens with 31 residues on the cytoplasmic side of the plasma membrane to mutants with only four residues in the cytoplasmic domain. This result limits ways in which the lateral diffusion of a major histocompatibility antigen, a transmembrane protein, can be constrained by interactions with other molecules.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal Biological Transport Cell Membrane/metabolism Diffusion H-2 Antigens/genetics,metabolism Histocompatibility Antigen H-2D Kinetics L Cells/metabolism Mice Mutation
Chemicals
Antibodies, Monoclonal H-2 Antigens Histocompatibility Antigen H-2D
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Edidin M
Zuniga M
References (32)
32 references, click to expand
  1. Lateral diffusion of rhodopsin in the photoreceptor membrane.
    Nature. 1974 Feb 15;247(5441):438-41 PMID: 4818543
  2. Lateral diffusion of visual pigment in photorecptor disk membranes.
    Science. 1974 Aug 2;185(4149):457-9 PMID: 4546260
  3. Brownian motion in biological membranes.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):3111-3 PMID: 1059096
  4. An association between actin and the major histocompatibility antigen H-2.
    Nature. 1978 May 25;273(5660):274-8 PMID: 565878
  5. Lateral diffusion of surface immunoglobulin, Thy-1 antigen, and a lipid probe in lymphocyte plasma membranes.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5163-7 PMID: 291930
  6. Rotational and lateral diffusion of membrane proteins.
    Biochim Biophys Acta. 1979 Dec 20;559(4):289-327 PMID: 391281
  7. Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain.
    Cell. 1980 Jun;20(2):303-12 PMID: 6771019
  8. Lateral mobility of integral membrane proteins is increased in spherocytic erythrocytes.
    Nature. 1980 Jun 12;285(5765):510-1 PMID: 7402296
  9. Diffusion, patching, and capping of stearoylated dextrans on 3T3 cell plasma membranes.
    Biochemistry. 1980 Aug 19;19(17):3893-904 PMID: 6157402
  10. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex.
    J Immunol. 1980 Dec;125(6):2473-7 PMID: 7191868
  11. Lateral distribution and diffusion of the C3b receptor of complement, HLA antigens, and lipid probes in peripheral blood leukocytes.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6587-91 PMID: 6935669
  12. Lateral diffusion of rhodopsin in photoreceptor cells measured by fluorescence photobleaching and recovery.
    Biophys J. 1981 Feb;33(2):225-32 PMID: 6971659
  13. Matrix control of protein diffusion in biological membranes.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3576-80 PMID: 6943558
  14. Purification of HLA-A2 antigen, fluorescent labeling of its intracellular region, and demonstration of an interaction between fluorescently labeled HLA-A2 antigen and lymphoblastoid cell cytoskeleton proteins in vitro.
    Biochemistry. 1981 Sep 15;20(19):5625-33 PMID: 6975122
  15. Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints.
    J Cell Biol. 1982 Jan;92(1):207-12 PMID: 7199052
  16. Molecular mobility on the cell surface.
    Biochem Soc Symp. 1981;(46):191-205 PMID: 7039623
  17. H-2Kk and vesicular stomatitis virus G proteins are not extensively associated in reconstituted membranes recognized by T cells.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1506-10 PMID: 6280187
  18. Extraction of membrane microviscosity from translational and rotational diffusion coefficients.
    Biophys J. 1982 Mar;37(3):673-6 PMID: 7074193
  19. Cell surface properties of HLA antigens on Epstein-Barr virus-transformed cell lines.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):608-12 PMID: 6281776
  20. Lateral and rotational diffusion of bacteriorhodopsin in lipid bilayers: experimental test of the Saffman-Delbrück equations.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4317-21 PMID: 6956861
  21. Unconstrained lateral diffusion of concanavalin A receptors on bulbous lymphocytes.
    Proc Natl Acad Sci U S A. 1982 Aug;79(16):4962-6 PMID: 6956904
  22. Lateral diffusion of H-2 antigens on mouse fibroblasts.
    J Cell Biol. 1982 Nov;95(2 Pt 1):458-62 PMID: 6815209
  23. Size dependence of the translational diffusion of large integral membrane proteins in liquid-crystalline phase lipid bilayers. A study using fluorescence recovery after photobleaching.
    Biochemistry. 1982 Oct 26;21(22):5608-12 PMID: 6216914
  24. International workshop on the application of fluorescence photobleaching techniques to problems in cell biology.
    Fed Proc. 1983 Jan;42(1):72-9 PMID: 6848381
  25. Lateral diffusion and patch formation of H-2Kk antigens on mouse spleen lymphocytes.
    Biochim Biophys Acta. 1983 Apr 5;762(2):281-8 PMID: 6600938
  26. Structure of class I major histocompatibility antigens.
    Contemp Top Mol Immunol. 1983;9:1-63 PMID: 6191921
  27. Expression and function of transplantation antigens with altered or deleted cytoplasmic domains.
    Cell. 1983 Sep;34(2):535-44 PMID: 6604582
  28. Ligand-induced association of rat lymphocyte membrane proteins with the detergent-insoluble lymphocyte cytoskeletal matrix.
    J Immunol. 1983 Oct;131(4):1917-9 PMID: 6604752
  29. Mobility modulation by local concanavalin A binding. Selectivity toward different membrane proteins.
    J Biol Chem. 1984 Feb 10;259(3):1515-9 PMID: 6693421
  30. Construction, expression and recognition of an H-2 molecule lacking its carboxyl terminus.
    Nature. 1984 Feb 2-8;307(5950):432-6 PMID: 6363940
  31. Components of the plasma membrane of growing axons. II. Diffusion of membrane protein complexes.
    J Cell Biol. 1984 Apr;98(4):1434-43 PMID: 6609163
  32. Genes of the major histocompatibility complex of the mouse.
    Annu Rev Immunol. 1983;1:529-68 PMID: 6152713
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.99.6.2333
Published
1984-12-00
Pages
2333-5
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113553
Subset
IM
Grants
NIAID NIH HHS · AI-14584 · United States
External Links
PubMed source DOI: 10.1083/jcb.99.6.2333 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2