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PMID: 6478036 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Agonists block currents through acetylcholine receptor channels.

Biophysical journal ·Vol. 46 ·No. 2 ·1984-08-00 ·Pages 277-83

Sine SM, Steinbach JH

Abstract

We have examined the effects of high concentrations of cholinergic agonists on currents through single acetylcholine receptor (AChR) channels on clonal BC3H1 cells. We find that raised concentrations of acetylcholine (ACh; above 300 microM) or carbamylcholine (Carb; above 1,000 microM) produce a voltage- and concentration-dependent reduction in the mean single-channel current. Raised concentrations of suberyldicholine (Sub; above 3 microM) produce a voltage- and concentration-dependent increase in the number of brief duration low-conductance interruptions of open-channel currents. These observations can be quantitatively described by a model in which agonist molecules enter and transiently occlude the ion-channel of the AChR.

MeSH Terms
Acetylcholine/pharmacology Animals Carbachol/pharmacology Cell Line Choline/analogs & derivatives,pharmacology Clone Cells Electric Conductivity/drug effects Membrane Potentials/drug effects Mice Receptors, Cholinergic/drug effects,physiology
Chemicals
Receptors, Cholinergic subecholine Carbachol Choline Acetylcholine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sine S M
Steinbach J H
References (17)
17 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(84)84022-9
Published
1984-08-00
Pages
277-83
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1435044
Subset
IM
Grants
NINDS NIH HHS · NS-13719 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(84)84022-9 PMC Full Text View journal details
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